Department of Environmental Health Sciences, School of Public Health and Health Sciences, University of Massachusetts, 173A Goessmann, 686 North Pleasant Street, Amherst MA 01003, USA.
Department of Biostatistics and Epidemiology, School of Public Health and Health Sciences, University of Massachusetts, 715 North Pleasant Street, Amherst MA 01003, USA.
Reprod Biomed Online. 2019 Jan;38(1):66-75. doi: 10.1016/j.rbmo.2018.10.004. Epub 2018 Nov 16.
To examine associations between sperm mitochondrial DNA copy number (mtDNAcn), sperm mitochondrial DNA deletions (mtDNAdel), semen parameters and clinical infertility in an IVF setting.
A total of 125 sperm samples were collected from men undergoing assisted reproductive procedures in an IVF clinic in Western Massachusetts, USA. Sperm mtDNAcn and mtDNAdel were measured by probe-based quantitative polymerase chain reaction. Semen parameters, clinical diagnoses of infertility, and infertility based on consecutive semen parameters, were fitted with mtDNAcn and mtDNAdel in linear models. The utility of sperm mtDNAcn and mtDNAdel to predict infertility was assessed by receiver operating characteristic curves.
Adjusting for relevant covariates, both sperm mtDNAcn and mtDNAdel were associated with lower sperm concentration, count, motility and morphology (P ≤ 0.03). Sperm mtDNAcn and mtDNAdel were also associated with increased risks of clinical infertility based on current and consecutive semen samples. Sperm mtDNAcn had high predictive accuracy for consecutive diagnoses of clinical infertility (C-statistic: 0.91), whereas sperm mtDNAdel had moderate predictive accuracy (C-statistic: 0.75).
Sperm mtDNAcn is a measure of consecutive abnormal semen parameters and has promise as a diagnostic test.
在美国马萨诸塞州西部的一家试管婴儿诊所，对 125 名接受辅助生殖程序的男性的精子线粒体 DNA 拷贝数 (mtDNAcn)、精子线粒体 DNA 缺失 (mtDNAdel)、精液参数与临床不孕不育之间的关联进行检测。
从美国马萨诸塞州西部的一家试管婴儿诊所接受辅助生殖程序的男性中收集了总共 125 个精子样本。通过基于探针的定量聚合酶链反应来测量精子 mtDNAcn 和 mtDNAdel。通过线性模型将精液参数、不孕不育的临床诊断以及基于连续精液参数的不孕不育与 mtDNAcn 和 mtDNAdel 进行拟合。通过接收者操作特征曲线来评估精子 mtDNAcn 和 mtDNAdel 预测不孕不育的效用。
在调整了相关协变量后，精子 mtDNAcn 和 mtDNAdel 均与精子浓度、计数、活力和形态降低有关（P ≤ 0.03）。精子 mtDNAcn 和 mtDNAdel 也与基于当前和连续精液样本的临床不孕不育风险增加有关。精子 mtDNAcn 对连续诊断临床不孕不育具有很高的预测准确性（C 统计量：0.91），而精子 mtDNAdel 具有中等预测准确性（C 统计量：0.75）。
精子 mtDNAcn 是连续异常精液参数的一种衡量标准，具有作为诊断测试的潜力。
