Galan-Valiente J, Soto-Otero R, Sierra-Marcuño G
Department of Biochemistry and Molecular Biology, School of Medicine, San Francisco.
Biomed Chromatogr. 1989 Mar;3(2):49-52. doi: 10.1002/bmc.1130030202.
A simple rapid method for simultaneous ethosuximide and phenobarbital assay in brain tissue, serum and urine has been developed. Extraction of samples from brain tissue and serum were performed with dichloromethane at low pH in the presence of an excess of ammonium sulfate. Glucuronide conjugates in urine samples were hydrolyzed by enzymatic cleavage with beta-glucuronidase and then extracted with dichloromethane. The extracts were analyzed using a Spherisorb 5 ODS column and a mixture of acetonitrile, methanol and phosphate buffer (21:24:55, v/v) as eluent. No interference was encountered and the method is both precise and reproducible.
已开发出一种用于同时测定脑组织、血清和尿液中乙琥胺和苯巴比妥的简单快速方法。在过量硫酸铵存在下，于低pH值条件下用二氯甲烷从脑组织和血清中提取样品。尿液样品中的葡萄糖醛酸缀合物用β-葡萄糖醛酸酶进行酶解，然后用二氯甲烷提取。提取物使用Spherisorb 5 ODS柱进行分析，并用乙腈、甲醇和磷酸盐缓冲液（21:24:55，v/v）的混合物作为洗脱剂。未遇到干扰，该方法精确且可重复。
