Soto-Otero R, Mendez-Alvarez E, Sierra-Paredes G, Galan-Valiente J, Aguilar-Veiga E, Sierra-Marcuño G
University of Santiago, Department of Biochemistry and Molecular Biology, School of Medicine, Spain.
Biomed Chromatogr. 1987;2(4):177-9. doi: 10.1002/bmc.1130020411.
A simple procedure for the determination of benzylpenicillin in serum is described. The assay involves the extraction of the drug and the internal standard (phenoxymethylpenicillinic acid) from the sample into dichloromethane, using tetrabutylammonium hydrogen sulfate neutralized with NaOH and buffered with citrate as an ion-pairing reagent. RP-HPLC was performed on a Spherisorb 5 ODS column, eluting the drugs isocratically with 14% acetonitrile in 10 mM ammonium acetate buffer. Monitoring was by UV detection at 208 nm. Our results show that the method is accurate and reproducible, permitting quantification of serum levels of benzylpenicillin without interference from other drugs commonly used in therapy. Analytical recovery was greater than 79.2%.
本文描述了一种测定血清中苄青霉素的简单方法。该测定方法包括使用用氢氧化钠中和并用柠檬酸盐缓冲的硫酸氢四丁铵作为离子对试剂，从样品中提取药物和内标（苯氧甲基青霉素酸）到二氯甲烷中。在Spherisorb 5 ODS柱上进行反相高效液相色谱，用10 mM醋酸铵缓冲液中14%的乙腈等度洗脱药物。通过在208 nm处的紫外检测进行监测。我们的结果表明，该方法准确且可重复，能够定量血清中苄青霉素的水平，而不受治疗中常用的其他药物的干扰。分析回收率大于79.2%。
