Department of Entomology, The University of Georgia, 120 Cedar Street, 420 Biological Sciences, Athens, GA, 30602, USA.
Parasit Vectors. 2016 Jun 30;9(1):375. doi: 10.1186/s13071-016-1660-9.
Aedes aegypti and A. atropalpus are related mosquitoes that differ reproductively. Aedes aegypti must blood-feed to produce eggs (anautogenous) while A. atropalpus always produces a first clutch of eggs without blood-feeding (facultatively autogenous). We recently characterized the gut microbiota of A. aegypti and A. atropalpus that were reared identically in the laboratory. Here, we assessed the effects of specific members of the gut microbiota in A. aegypti and A. atropalpus on female fitness including egg production.
Gnotobiotic A. aegypti and A. atropalpus larvae were colonized by specific members of the gut microbiota. Survival, development time, size and egg production for each treatment was then compared to axenic and conventionally reared larvae.
Most species of bacteria we tested supported normal development and egg production by A. aegypti but only one betaproteobacterium, a Comamonas, supported development and egg production by A. atropalpus to equivalent levels as conventionally reared females. Aedes atropalpus females colonized by Comamonas contained similar stores of glycogen and protein as conventionally reared females, whereas females colonized by Aquitalea did not. Small differences in bacterial loads were detected between gnotobiotic and conventionally reared A. aegypti and A. atropalpus, but this variation did not correlate with the beneficial effects of Comamonas in A. atropalpus.
Specific members of the gut microbiota more strongly affected survival, size and egg production by A. atropalpus than A. aegypti.
埃及伊蚊和 A. atropalpus 是相关的蚊子，它们在繁殖上有所不同。埃及伊蚊必须吸血才能产卵（自育性），而 A. atropalpus 总是在不吸血的情况下产生第一批卵（兼性自育性）。我们最近对在实验室中以相同方式饲养的埃及伊蚊和 A. atropalpus 的肠道微生物群进行了特征描述。在这里，我们评估了肠道微生物群中特定成员对埃及伊蚊和 A. atropalpus 雌性适合度的影响，包括产卵。
无菌 A. aegypti 和 A. atropalpus 幼虫被肠道微生物群的特定成员定植。然后比较每种处理的存活率、发育时间、大小和产卵量与无菌和常规饲养的幼虫。
我们测试的大多数细菌物种都支持埃及伊蚊的正常发育和产卵，但只有一种β变形菌，即 Comamonas，支持 A. atropalpus 的发育和产卵，与常规饲养的雌性相当。被 Comamonas 定植的 A. atropalpus 雌性体内的糖原和蛋白质储存与常规饲养的雌性相似，而被 Aquitalea 定植的雌性则没有。在无菌和常规饲养的 A. aegypti 和 A. atropalpus 之间检测到细菌负荷的微小差异，但这种差异与 Comamonas 在 A. atropalpus 中的有益作用无关。
肠道微生物群的特定成员对 A. atropalpus 的存活、大小和产卵的影响大于 A. aegypti。
