Reddy Varikasuvu Seshadri, Sethi Sumita, Agrawal Poonam, Gupta Navdeep, Garg Renu
Nepal J Ophthalmol. 2015 Jul;7(14):117-23. doi: 10.3126/nepjoph.v7i2.14960.
Oxidative stress has important role in the pathophysiology of diabetic retinopathy (DR). Ischemia modified albumin (IMA) has been recently considered as a marker of oxidative damage in diabetes. However, there is scarcity of published information about both IMA and albumin adjusted-IMA (AAIMA) in DR patients.
To evaluate the serum levels of IMA and AAIMA in patients with DR and in healthy controls.
This was a cross sectional study. Serum was obtained to measure lipids, albumin and IMA from the the patients with DR and non-diabetic subjects. The IMA level was measured by a colorimetric albumin cobalt binding (ACB) assay and the values were presented as absorbance units (ABSU). The IMA levels were adjusted for albumin interference and the AAIMA by using a formula [Individual serum albumin/median albumin concentration of the population X IMA].
This study was done on 18 DR and 20 non- diabetic patients. The mean Serum IMA values in DR group and controls were 0.50±0.17 and 0.32±0.17, respectively (P=0.002). The mean serum AAIMA values in DR group and controls were 0.48±0.20 and 0.32±0.17, respectively (P=0.01). The albumin and HDL- Cholesterol levels were significantly lower in DR patients compared to controls (p=0.004 and p=0.01, respectively).
The level of IMA and AAIMA were higher in cases of DR compared to that of non-diabetic subjects. The levels of albumin and HDL-Cholesterol were lower in DR patients compared to controls.
氧化应激在糖尿病视网膜病变（DR）的病理生理学中起重要作用。缺血修饰白蛋白（IMA）最近被认为是糖尿病氧化损伤的一个标志物。然而，关于DR患者中IMA和白蛋白校正IMA（AAIMA）的已发表信息很少。
评估DR患者和健康对照者血清中IMA和AAIMA的水平。
这是一项横断面研究。采集DR患者和非糖尿病受试者的血清以测量血脂、白蛋白和IMA。IMA水平通过比色白蛋白钴结合（ACB）测定法测量，结果以吸光度单位（ABSU）表示。通过使用公式[个体血清白蛋白/人群中白蛋白中位数浓度×IMA]对IMA水平进行白蛋白干扰校正并得出AAIMA。
本研究纳入了18例DR患者和20例非糖尿病患者。DR组和对照组的血清IMA平均值分别为0.50±0.17和0.32±0.17（P = 0.002）。DR组和对照组的血清AAIMA平均值分别为0.48±0.20和0.32±0.17（P = 0.01）。与对照组相比，DR患者的白蛋白和高密度脂蛋白胆固醇水平显著降低（分别为p = 0.004和p = 0.01）。
与非糖尿病受试者相比，DR患者的IMA和AAIMA水平更高。与对照组相比，DR患者的白蛋白和高密度脂蛋白胆固醇水平更低。
