高脂高能量饮食影响低出生体重发育中雌性大鼠的肝脏CYP3A表达及活性。
A high-fat high-energy diet influences hepatic CYP3A expression and activity in low-birth-weight developing female rats.
作者信息
Ni Shao-Qing, Lou Yin, Wang Xiu-Min, Shen Zheng, Wang Jue, Zhao Zheng-Yan, Zeng Su
机构信息
The National Clinical Trial Institute (Ni SQ), Pharmacy Department, Zhejiang University School of Medicine and Zhejiang Key Laboratory for Diagnosis and Therapy of Neonatal Diseases, Hangzhou, China.
Department of Endocrinology, Zhejiang University School of Medicine and Zhejiang Key Laboratory for Diagnosis and Therapy of Neonatal Diseases, Hangzhou, China.
出版信息
World J Pediatr. 2016 Nov;12(4):489-497. doi: 10.1007/s12519-016-0019-9. Epub 2016 Jun 30.
BACKGROUND
The objective of this study was to investigate the effects of a high-fat, high-energy (HFHE) diet on the hepatic expression of CYP3A in low-birthweight developing female rats.
METHODS
Pregnant rats were divided into nourished and undernourished groups. The offspring of the nourished rats were defined as the normal-birth-weight (NBW) group, and those of undernourished rats were defined as the low-birth-weight (LBW) group. According to their birth weights and diets, the rats were subdivided into the following four groups: NBW-normal diet (NN) group; NBW-HFHE (NH) group; LBW-normal diet (LN) group; and LBW-HFHE (LH) group. Liver samples were isolated on days 3, 7, 14, 21, 28, 56 and 84 after birth.
RESULTS
The CYP3A1 mRNA levels in the LH group on days 3, 56 and 84 were significantly higher than those of the NN group (P<0.05). CYP3A1 expression was significantly higher in the LH group than that in the NH group on days 21, 28 and 84 (P<0.05). CYP3A1 mRNA expression was higher in the LH group than that in the LN group on days 3 and 21 (P<0.05). No zonal CYP3A1 expression pattern was observed in the LH developmental group. The LH group had significantly higher mean activity than the LN group on days 7, 14, 28 and 56.
CONCLUSION
Our results indicated that an HFHE diet can result in alterations of CYP3A expression in a developmental LBW rat model.
背景
本研究的目的是调查高脂、高能量（HFHE）饮食对低出生体重发育中雌性大鼠肝脏中CYP3A表达的影响。
方法
将怀孕大鼠分为营养充足组和营养不足组。营养充足组的后代定义为正常出生体重（NBW）组，营养不足组的后代定义为低出生体重（LBW）组。根据出生体重和饮食情况，将大鼠再细分为以下四组：NBW-正常饮食（NN）组；NBW-HFHE（NH）组；LBW-正常饮食（LN）组；LBW-HFHE（LH）组。在出生后第3、7、14、21、28、56和84天采集肝脏样本。
结果
LH组在出生后第3、56和84天的CYP3A1 mRNA水平显著高于NN组（P<0.05）。在出生后第21、28和84天，LH组的CYP3A1表达显著高于NH组（P<0.05）。在出生后第3和21天，LH组的CYP3A1 mRNA表达高于LN组（P<0.05）。在LH发育组中未观察到CYP3A1的区域表达模式。在出生后第7、14、28和56天，LH组的平均活性显著高于LN组。
结论
我们的结果表明，HFHE饮食可导致发育中的低出生体重大鼠模型中CYP3A表达发生改变。
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