Alp Harun, Pinar Neslihan, Dokuyucu Recep, Sahan Mustafa, Oruc Cem, Kaplan Ibrahim, Senol Serkan, Ceyran Ayse Bahar
Department of Pharmacology, School of Medicine, Mustafa Kemal University, Hatay, Turkey.
Department of Medical Physiology, School of Medicine, Mustafa Kemal University, Hatay, Turkey.
Biochem Genet. 2016 Dec;54(6):803-815. doi: 10.1007/s10528-016-9757-6. Epub 2016 Jun 30.
The present study was aimed to the investigate the protective effects of caffeic acid phenethyl ester (CAPE) and intralipid (IL) on hepatotoxicity and pancreatic injury caused by acute dichlorvos (D) intoxication in rats. Forty-eight Wistar rats were randomly divided into seven groups each containing seven rats except control groups. The groups included control, D, CAPE, IL, D + CAPE, D + IL, and D + CAPE + IL. Total antioxidant status and total oxidative stress levels were measured by automated colorimetric assay. Tissues were evaluated using hematoxylin and eosin (H&E) staining. Tissues were analyzed with hematoxylin and eosin by using standard protocols. Also, Bcl-2, Bax and caspase-3 were evaluated by immunohistochemical method in liver tissue. Total oxidant status in control, CAPE, and IL groups were significantly lower, and total antioxidant status in the D + CAPE, D + IL, and D + IL + CAPE groups were significantly higher compared to the D group. CAPE and IL treatment decreased the apoptotic and mitotic cell count in liver tissue. Parenchymal necrosis caused by dichlorvos is observed in pancreas tissues of rats. Mild congestion and edema formation occurred in pancreas tissues following D + CAPE and D + IL therapies. These results indicate that CAPE and IL have the potential to decrease oxidative stress and hepatic and pancreatic injuries caused by acute dichlorvos intoxication. These drugs can be considered as a new method for supportive and protective therapy against pesticide intoxication.
本研究旨在探讨咖啡酸苯乙酯（CAPE）和脂肪乳剂（IL）对大鼠急性敌敌畏（D）中毒所致肝毒性和胰腺损伤的保护作用。48只Wistar大鼠被随机分为七组，每组七只，对照组除外。这些组包括对照组、D组、CAPE组、IL组、D + CAPE组、D + IL组和D + CAPE + IL组。通过自动比色法测定总抗氧化状态和总氧化应激水平。使用苏木精和伊红（H&E）染色对组织进行评估。按照标准方案用苏木精和伊红对组织进行分析。此外，通过免疫组织化学方法评估肝组织中的Bcl-2、Bax和半胱天冬酶-3。与D组相比，对照组、CAPE组和IL组的总氧化剂状态显著降低，而D + CAPE组、D + IL组和D + IL + CAPE组的总抗氧化状态显著升高。CAPE和IL治疗降低了肝组织中的凋亡和有丝分裂细胞计数。在大鼠胰腺组织中观察到敌敌畏引起的实质坏死。D + CAPE和D + IL治疗后胰腺组织出现轻度充血和水肿形成。这些结果表明，CAPE和IL有可能降低急性敌敌畏中毒引起的氧化应激以及肝脏和胰腺损伤。这些药物可被视为一种针对农药中毒的支持性和保护性治疗的新方法。
