Dokuyucu Recep, Bilgili Ali, Hanedan Basak, Dogan Hatice, Dokuyucu Ahmet, Celik Muhammed Murat
Mustafa Kemal University, Hatay, Turkey (Faculty of Medicine, Department of Physiology).
Ankara University, Ankara, Turkey (Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology).
Med Pr. 2016 Dec 22;67(6):743-749. doi: 10.13075/mp.5893.00462. Epub 2016 Dec 12.
Chlorpyrifos (CPF), insecticide widely used in agriculture, may cause poisonings in the case of humans. As a result, there is a large amount of treatment research underway to focus on the possibility of chlorpyrifos induced poisonings. The aim of this study has been to evaluate the effects of caffeic acid phenethyl ester (CAPE) and intralipid (IL) on hepatotoxicity induced by chlorpyrifos in the case of rats.
The rats in this study were treated with CPF (10 mg/kg body weight (b.w.), orally), CAPE (10 μmol/kg b.w., intraperitoneally), IL (18.6 ml/kg b.w., orally), CPF+CAPE, CPF+IL, and CPF+CAPE+IL. The plasma total oxidant capacity (TOC), total antioxidant capacity (TAC) were measured and the oxidative stress index (OSI) was calculated. Liver histopathology and immunohistochemical staining were performed.
Chlorpyrifos statistically significantly decreased the TAC levels in the rats' plasma and increased the apoptosis and the TOC and OSI levels. In the chlorpyrifos induced liver injury, CAPE and CAPE+IL significantly decreased the plasma OSI levels and the apoptosis, and significantly increased the plasma TAC levels.
This study revealed that CAPE and CAPE+IL attenuate chlorpyrifos induced liver injuries by decreasing oxidative stress and apoptosis. Med Pr 2016;67(6):743-749.
毒死蜱（CPF）是一种广泛应用于农业的杀虫剂，可导致人类中毒。因此，目前正在进行大量治疗研究，重点关注毒死蜱中毒的可能性。本研究的目的是评估咖啡酸苯乙酯（CAPE）和脂肪乳剂（IL）对大鼠毒死蜱诱导的肝毒性的影响。
本研究中的大鼠分别接受毒死蜱（10毫克/千克体重，口服）、咖啡酸苯乙酯（10微摩尔/千克体重，腹腔注射）、脂肪乳剂（18.6毫升/千克体重，口服）、毒死蜱+咖啡酸苯乙酯、毒死蜱+脂肪乳剂以及毒死蜱+咖啡酸苯乙酯+脂肪乳剂处理。测定血浆总氧化能力（TOC）、总抗氧化能力（TAC），并计算氧化应激指数（OSI）。进行肝脏组织病理学和免疫组织化学染色。
毒死蜱使大鼠血浆中的TAC水平显著降低，使细胞凋亡以及TOC和OSI水平升高。在毒死蜱诱导的肝损伤中，咖啡酸苯乙酯和咖啡酸苯乙酯+脂肪乳剂显著降低血浆OSI水平和细胞凋亡，并显著提高血浆TAC水平。
本研究表明，咖啡酸苯乙酯和咖啡酸苯乙酯+脂肪乳剂通过降低氧化应激和细胞凋亡减轻毒死蜱诱导的肝损伤。《医学实践》2016年；67(6):743 - 749。
