Shen Zhi-Gang, Wang Han-Ping, Yao Hong, O'Bryant Paul, Rapp Dean, Zhu Kun-Qian
Aquaculture Genetics and Breeding Laboratory, The Ohio State University South Centers, 1864 Shyville Road, Piketon, Ohio, 45661.
Aquaculture Genetics and Breeding Laboratory, The Ohio State University South Centers, 1864 Shyville Road, Piketon, Ohio, 45661
Biol Bull. 2016 Jun;230(3):197-208. doi: 10.1086/BBLv230n3p197.
There is increasing evidence that temperature effects on sex ratio in fish species are ubiquitous. Temperature effects on sex ratio could be influenced by parent, strain, and population, whether in fish species with temperature-dependent sex determination or genetic sex determination plus temperature effects. In the present study, effects of genotype-temperature interactions on sex determination in bluegill sunfish were further investigated, based on our previous results, using four geographic strains: Hebron, Jones, Hocking, and Missouri. In the Hebron strain, the two higher-temperature treatment groups (24 °C and 32 °C) produced more males than the low-temperature treatment group (17 °C) from 6 days post-hatching (dph) to 90 dph. In contrast, the low-temperature treatment produced more males than the other two higher-temperature treatments in the Jones strain. No significant effects of temperature on sex ratio were detected in the other two strains. Our results from sex ratio variance in different treatment times suggest that the thermosensitive period of sex differentiation occurs prior to 40 dph. Our results further confirmed that genotype-temperature interactions influence sex determination in bluegill. Therefore, to significantly increase the proportion of males, which grow faster and larger than females, a consumer- and environment-friendly approach may be achieved through selection of temperature sensitivity in bluegill.
越来越多的证据表明，温度对鱼类性别比例的影响是普遍存在的。温度对性别比例的影响可能受到亲本、品系和种群的影响，无论是在具有温度依赖性性别决定的鱼类物种中，还是在遗传性别决定加上温度影响的鱼类物种中。在本研究中，基于我们之前的结果，使用四个地理品系：希伯伦、琼斯、霍金和密苏里，进一步研究了基因型 - 温度相互作用对蓝鳃太阳鱼性别决定的影响。在希伯伦品系中，从孵化后6天（dph）到90 dph，两个较高温度处理组（24°C和32°C）产生的雄性比低温处理组（17°C）更多。相比之下，在琼斯品系中，低温处理产生的雄性比其他两个较高温度处理更多。在其他两个品系中未检测到温度对性别比例的显著影响。我们在不同处理时间的性别比例方差结果表明，性别分化的热敏期发生在40 dph之前。我们的结果进一步证实了基因型 - 温度相互作用影响蓝鳃太阳鱼的性别决定。因此，为了显著提高生长速度比雌性更快、体型比雌性更大的雄性比例，可以通过选择蓝鳃太阳鱼的温度敏感性来实现一种对消费者和环境友好的方法。
