Gupta M K, Misra R N, Chawla N, Mani H, Chowdhry C N, Singh S P, Gupta S
Senior Advisor (Pathology & Haematology), INHS Asvini, Colaba, Mumbai-400 005.
Classified Specialist (Pathology), INHS Asvini, Colaba, Mumbai-400 005.
Med J Armed Forces India. 2001 Jul;57(3):188-90. doi: 10.1016/S0377-1237(01)80039-3. Epub 2011 Jul 21.
75 patients with clinical features suggestive of malaria were studied to evaluate the efficacy of immunochromatographic test (ICT), which detects histidine rich protein-2 antigen secreted by Plasmodium falciparum (Pfhrp-2), as against direct microscopy. There were 40 cases of P falciparum malaria, 14 cases of P vivax malaria and 21 cases of non-malarial fevers. Direct microscopy could detect 27(67.5%) P falciparum cases but failed to detect 13 cases (32.5%) whereas ICT could detect 35(87.5%) P falciparum cases out of 40 but failed to detect 5(12.5%) cases. All the P vivax cases and non-malarial fever cases were negative for ICT. The sensitivity and specificity of ICT is 87.5% and 100% respectively where as the positive predictive value and the negative predictive value of the test is 100% and 87.5% respectively. It is concluded that ICT test is a good adjunct to blood smear studies in fever cases with neurological and multiorgan dysfunction and in antenatal ladies.
对75例具有疟疾临床特征的患者进行了研究，以评估免疫层析试验（ICT）检测恶性疟原虫分泌的富含组氨酸蛋白-2抗原（Pfhrp-2）相对于直接显微镜检查的有效性。其中有40例恶性疟病例、14例间日疟病例和21例非疟疾发热病例。直接显微镜检查能检测出27例（67.5%）恶性疟病例，但有13例（32.5%）未检测出；而ICT在40例恶性疟病例中能检测出35例（87.5%），有5例（12.5%）未检测出。所有间日疟病例和非疟疾发热病例的ICT检测均为阴性。ICT的敏感性和特异性分别为87.5%和100%，而该检测的阳性预测值和阴性预测值分别为100%和87.5%。结论是，在伴有神经和多器官功能障碍的发热病例以及产前女性中，ICT检测是血液涂片检查的良好辅助手段。
