Galate Lata Baswanna, Agrawal Sachee R, Shastri Jayanthi S, Londhey Vikram
Department of Microbiology, GMC, Akola, Maharashtra, India.
Department of Microbiology, TNMC and BYL, Nair Charitable Hospital, Mumbai, Maharashtra, India.
J Lab Physicians. 2016 Jul-Dec;8(2):85-9. doi: 10.4103/0974-2727.180787.
Chikungunya fever (CHIK) is an arboviral disease. Dengue fever (DENG) and CHIK are indistinguishable clinically and need to be differentiated by laboratory investigations.
This study aimed at estimating the seroprevalence of CHIK mono-infection and CHIK and DENG dual infection in suspected patients. We also analyzed the age, sex distribution, joint involvement, and relation of joint movement restriction with visual analog scale (VAS).
Two hundred patients clinically suspected with DENG and CHIK were enrolled from a Tertiary Care Hospital in Mumbai from April 2012 to October 2013. The detailed history and examination findings were recorded. Serum samples were subjected to DENG and CHIK immunoglobulin G (IgM) enzyme-linked immunosorbent assay (ELISA).
The seroprevalence of CHIK was 12.5%. Mono-infection of CHIK was 3%, and CHIK and DENG dual infection was 9.5%. Most affected age group in CHIK cases was 46-60 years wherein female preponderance was seen. All 6 patients with CHIK mono-infection had fever and joint involvement; knee and elbow were the most commonly affected joints. All CHIK patients had VAS score of 6-10 with restricted joint movement. Of the patients with dual infection, the majorities were from 31 to 45 years with male preponderance; all had fever and joint pain mainly affecting knee and elbow. Of patients who had VAS score 6-10 in patients with dual infection, only 5.26% had restricted joint movement.
IgM ELISA for Chikungunya infection should be included in the routine laboratory tests for acute febrile illness.
基孔肯雅热（CHIK）是一种虫媒病毒病。登革热（DENG）和基孔肯雅热在临床上难以区分，需要通过实验室检查来鉴别。
本研究旨在评估疑似患者中基孔肯雅热单一感染以及基孔肯雅热和登革热双重感染的血清流行率。我们还分析了年龄、性别分布、关节受累情况以及关节活动受限与视觉模拟评分（VAS）的关系。
2012年4月至2013年10月期间，从孟买一家三级护理医院招募了200名临床疑似感染登革热和基孔肯雅热的患者。记录详细的病史和检查结果。血清样本进行登革热和基孔肯雅热免疫球蛋白G（IgM）酶联免疫吸附测定（ELISA）。
基孔肯雅热的血清流行率为12.5%。基孔肯雅热单一感染率为3%，基孔肯雅热和登革热双重感染率为9.5%。基孔肯雅热病例中受影响最严重的年龄组为46 - 60岁，其中女性占优势。所有6例基孔肯雅热单一感染患者均有发热和关节受累；膝关节和肘关节是最常受累的关节。所有基孔肯雅热患者的VAS评分为6 - 10分，关节活动受限。在双重感染患者中，大多数年龄在31至45岁，男性占优势；所有人都有发热和关节疼痛，主要影响膝关节和肘关节。在双重感染患者中，VAS评分为6 - 10分的患者中，只有5.26%有关节活动受限。
基孔肯雅热感染的IgM ELISA应纳入急性发热性疾病的常规实验室检查中。
