Dash Raju, Emran Talha Bin, Paul Arkajyoti, Siddique Mohammad Kutub Uddin, Khan Mohammad Arfad, Rahman Md Golamur, Sarwar Md Shahid, Nasir Uddin Mir Muhammad
Department of Pharmacy, BGC Trust University, Chittagong-4000, Bangladesh, India.
Department of Pharmacy, BGC Trust University, Chittagong-4000, Bangladesh, India; Department of Biochemistry and Molecular Biology, University of Chittagong, Chittagong-4331, Bangladesh, India.
Pharmacognosy Res. 2016 Jul-Sep;8(3):176-80. doi: 10.4103/0974-8490.181403.
Piper Betle, Pterospermum acerifolium, Saraca indica, Argyreia speciosa, and Rhaphidophora glauca are medicinal plants commonly used as traditional medicine for the treatment of various diseases. To examine, whether organic extracts of these plants possess thrombolytic properties with minimal or no toxicity is our main aim of the study.
In vitro thrombolytic model was used to check the clot lysis effects using streptokinase (SK) as a positive control and water as a negative control. Cytotoxicity was screened by brine shrimp lethality bioassay using vincristine sulfate (VS) as positive control.
Among herbal drugs, different organic extracts of P. betle, P. acerifolium, S. indica, A. speciosa, and R. glauca showed significant (P < 0.05 and P < 0.0001) clot lysis activity viz., 31.58% ±0.76%, 40.50% ±0.94%, 49.70% ±1.69%, 35.81% ±0.86%, and 43.80% ±0.91%, respectively, compared to reference drug SK (79.32% ±1.629%). In brine shrimp cytotoxic assay, mortality achieved by the extracts showed lethal concentration 50 (LC50) values 274.64 ± 3.46, 215.60 ± 4.59, 478.40 ± 6.98, 233.37 ± 2.56, and 209.32 ± 1.98 μg/ml, respectively, with reference to VS (LC50, 0.05 ± 0.34).
In this study, S. indica, R. glauca, and P. acerifolium possessed effective thrombolytic activity. Further studies can be undertaken to identify certain structure of the ingredients in the extracts and to elucidate the precise mechanism of action.
Five Bangladesh medicinal plants, named Piper betle, Pterospermum acerifolium, Saraca indica, Argyreia speciosa, and Rhaphidophora glauca were subjected to comparative antithrombotic and toxicity based analysis. In comparative study, Saraca indica showed highest clot lysis (49.70 ± 1.69%) activity among the other plant with lowest toxicity (LC50: 478.40 ± 6.98) Abbreviations Used: h: Hour; min: Minutes; sec: Second; kg: Kilogram; g: Gram; μg: Microgram; L: Liter; mL: Millilitre; μL: Micro liter; μg/mL: Microgram per Milliliter; mg/kg: Milligram per kilogram; %: Percent; °C: Degree Celsius; et al.: et alliori (and others); w/w: Weight by Weight; v/v: Volume by Volume; SEM: Standard Error Mean; LC50: lethal concentration at 50.
蒌叶、枫香树、无忧花、美丽银背藤和绿麒麟尾是常用作治疗各种疾病的传统药物的药用植物。本研究的主要目的是检验这些植物的有机提取物是否具有溶栓特性且毒性最小或无毒性。
使用体外溶栓模型，以链激酶（SK）作为阳性对照，水作为阴性对照，检查凝块溶解效果。通过使用硫酸长春新碱（VS）作为阳性对照的卤虫致死生物测定法筛选细胞毒性。
在草药中，蒌叶、枫香树、无忧花、美丽银背藤和绿麒麟尾的不同有机提取物显示出显著（P < 0.05和P < 0.0001）的凝块溶解活性，分别为31.58% ±0.76%、40.50% ±0.94%、49.70% ±1.69%、35.81% ±0.86%和43.80% ±0.91%，与参考药物SK（79.32% ±1.629%）相比。在卤虫细胞毒性测定中，提取物的死亡率显示致死浓度50（LC50）值分别为274.64 ± 3.46、215.60 ± 4.59、478.40 ± 6.98、233.37 ± 2.56和209.32 ± 1.98 μg/ml，参考VS（LC50，0.05 ± 0.34）。
在本研究中，无忧花、绿麒麟尾和枫香树具有有效的溶栓活性。可以进行进一步的研究以确定提取物中成分的某些结构并阐明确切的作用机制。
对五种孟加拉国药用植物，即蒌叶、枫香树、无忧花、美丽银背藤和绿麒麟尾进行了基于抗血栓形成和毒性的比较分析。在比较研究中，无忧花在其他植物中显示出最高的凝块溶解（49.70 ± 1.69%）活性，毒性最低（LC50：478.40 ± 6.98）
h：小时；min：分钟；sec：秒；kg：千克；g：克；μg：微克；L：升；mL：毫升；μL：微升；μg/mL：微克每毫升；mg/kg：毫克每千克；%：百分比；°C：摄氏度；et al.：以及其他；w/w：重量比；v/v：体积比；SEM：标准误差均值；LC50：50%致死浓度
