Esmailidehaj Mansour, Rasulian Bahram, Rezvani Mohammad Ebrahim, Delfan Bahram, Mosaddeghmehrjardi Mohammad Hossein, Pourkhalili Khalil
Department of Physiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; Cardiovascular Research Center, Afshar Hospital, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
EXCLI J. 2012 Apr 3;11:150-62. eCollection 2012.
Previous studies have reported that oleuropein, the major constituent of olive leaves, has cardioprotective effects. There is no report related to oleuropein and ischemic-reperfusion injuries (cardiac dysfunction and myocardial infarction) as well as preconditioning in rat hearts. 56 male Wistar rats were divided into 7 groups (n=8). Group 1 as the control group and groups 2 to 7 as the treatment groups that received a single dose of oleuropein (100 mg/kg, i.p.) 1, 3, 6, 12, 24 and 48 hours before the excision of the heart, respectively. After these times, their hearts were excised and subjected to 30 min regional ischemia and 120 min reperfusion under Langendorff apparatus. Electrocardiogram and intraventricular pressures were monitored and recorded throughout the procedure. Finally, infarct size was measured by triphenyltetrazolium chloride staining. Compared to the control group, oleuropein significantly reduced infarct size and reperfusion-induced cardiac dysfunction in groups 2 and 3. Oleuropein markedly attenuated both ischemic and reperfusion arrhythmias in groups 2 and 3. There was no significant difference between other groups (4 to 7) than the control group. Heart rate had no significant difference among all of the groups. These results indicate that pretreatment of rats with a single dose of intraperitoneal oleuropein could protect their heart against ischemic-reperfusion injury for at least 3 hours. However, it has no preconditioning effect, since oleuropein had not cardioprotective effects 24 hour later.
先前的研究报道，橄榄叶的主要成分橄榄苦苷具有心脏保护作用。目前尚无关于橄榄苦苷与缺血再灌注损伤（心脏功能障碍和心肌梗死）以及大鼠心脏预处理的相关报道。56只雄性Wistar大鼠被分为7组（n = 8）。第1组作为对照组，第2至7组作为治疗组，分别在心脏切除前1、3、6、12、24和48小时接受单剂量的橄榄苦苷（100 mg/kg，腹腔注射）。在这些时间点之后，切除它们的心脏，并在Langendorff装置下进行30分钟的局部缺血和120分钟的再灌注。在整个过程中监测并记录心电图和心室内压。最后，通过氯化三苯基四氮唑染色测量梗死面积。与对照组相比，橄榄苦苷在第2组和第3组中显著减小了梗死面积并减轻了再灌注诱导的心脏功能障碍。橄榄苦苷在第2组和第3组中显著减轻了缺血性和再灌注性心律失常。与对照组相比，其他组（第4至7组）之间没有显著差异。所有组之间心率无显著差异。这些结果表明，用单剂量腹腔注射橄榄苦苷预处理大鼠可使其心脏至少在3小时内免受缺血再灌注损伤。然而，它没有预处理作用，因为24小时后橄榄苦苷没有心脏保护作用。
