Emerman M, Vazeux R, Peden K
Unité d'Oncologie Virale, Institut Pasteur, CNRS UA1157, Paris, France.
Cell. 1989 Jun 30;57(7):1155-65. doi: 10.1016/0092-8674(89)90053-6.
By in situ hybridization analysis and immunoprecipitations following transfection of COS cells, we show that the Rev protein of the human immunodeficiency virus is necessary for envelope protein expression, which is correlated with the appearance in the cytoplasm of envelope-specific RNA. In the absence of cotransfection with a plasmid expressing Rev, envelope-specific RNA is retained in the nucleus. Several cis-acting sites in the envelope are involved, one of which is between nucleotides 7330 and 7735 and is required for the response to Rev. Other sequences (nucleotides 5797-7330 and 7735-7989) are involved in the apparent retention of the envelope-specific RNA in the nucleus in the absence of Rev and its response element. Because Rev affects the localization of envelope RNA both in the presence and in the absence of the normal splice sites on the RNA, the mechanism of Rev action is independent of splicing.
通过对转染COS细胞后的原位杂交分析和免疫沉淀，我们发现人类免疫缺陷病毒的Rev蛋白是包膜蛋白表达所必需的，这与包膜特异性RNA在细胞质中的出现相关。在没有与表达Rev的质粒共转染的情况下，包膜特异性RNA保留在细胞核中。包膜中的几个顺式作用位点参与其中，其中一个位于核苷酸7330和7735之间，是对Rev作出反应所必需的。其他序列（核苷酸5797 - 7330和7735 - 7989）在没有Rev及其反应元件的情况下参与包膜特异性RNA在细胞核中的明显保留。由于Rev在RNA上存在和不存在正常剪接位点的情况下均影响包膜RNA的定位，因此Rev的作用机制与剪接无关。
