Hammarskjöld M L, Heimer J, Hammarskjöld B, Sangwan I, Albert L, Rekosh D
Department of Microbiology, State University of New York, Buffalo 14214.
J Virol. 1989 May;63(5):1959-66. doi: 10.1128/JVI.63.5.1959-1966.1989.
A single simian virus 40 late replacement vector which expresses both the rev and envelope (env) genes of human immunodeficiency virus was used to examine the mechanism underlying the dependence of env gene expression on the rev protein. When rev was deleted from the vector, no envelope protein expression could be detected in transfected cells, and the levels of cytoplasmic env mRNA were dramatically reduced. In contrast to this, the levels of env RNA in total cellular RNA preparations were similar with or without rev coexpression, and analysis of nuclear RNA showed that the levels of nuclear env RNA were increased in the absence of rev. These results suggest that rev functions to regulate nuclear export of env mRNA. It was possible to restore env expression from the vector lacking rev by supplying rev in trans, provided that a cis-acting sequence was also present. This sequence was mapped to a 854-base-pair region within the env open reading frame, and it was shown that the sequence could be moved but that it worked only in its original orientation.
使用一种表达人类免疫缺陷病毒的rev和包膜（env）基因的单一猿猴病毒40晚期替代载体来研究env基因表达对rev蛋白依赖性的潜在机制。当从载体中删除rev时，在转染细胞中检测不到包膜蛋白表达，并且细胞质env mRNA水平显著降低。与此相反，无论是否共表达rev，总细胞RNA制剂中env RNA的水平相似，并且对核RNA的分析表明，在没有rev的情况下核env RNA水平增加。这些结果表明，rev起到调节env mRNA核输出的作用。通过反式提供rev，可以从缺乏rev的载体中恢复env表达，前提是也存在顺式作用序列。该序列被定位到env开放阅读框内的一个854碱基对区域，并且表明该序列可以移动，但仅在其原始方向起作用。
