Nabizadeh Arezoo, Bamdad Taravat, Arefian Ehsan, Razavi Nikoo Seyed Hadi
Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran.
Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, IR Iran.
Iran J Cancer Prev. 2016 Feb 22;9(1):e3919. doi: 10.17795/ijcp-3919. eCollection 2016 Feb.
Beclin1 is an important, primary molecule for autophagy.
It is suggested that the control of the autophagy path increases the sensitivity of tumor cells to VSV.
In this study, the degree of Beclin1 gene expression in two cell lines, HeLa and A549, has been examined and the percentage of living cells subsequent infection with virus has been evaluated by MTT assay method.
The results showed that the degree of Beclin1 gene expression in HeLa cells in comparison with A549 cells has reduced, and the sensitivity of these cells to vesicular stomatits virus (VSV) oncolysis is more than A549.
It seems that by using some methods for reducing autophagy, it is possible to make tumor cells more sensitive to virotherapy and even other treatments.
Beclin1是自噬的一个重要的主要分子。
有人提出，控制自噬途径可增加肿瘤细胞对水泡性口炎病毒（VSV）的敏感性。
在本研究中，检测了HeLa和A549两种细胞系中Beclin1基因的表达程度，并通过MTT法评估了病毒感染后活细胞的百分比。
结果显示，与A549细胞相比，HeLa细胞中Beclin1基因的表达程度降低，且这些细胞对水泡性口炎病毒（VSV）溶瘤的敏感性高于A549细胞。
似乎通过使用一些降低自噬的方法，有可能使肿瘤细胞对病毒疗法甚至其他治疗更敏感。
