自噬基因活性可能是肿瘤细胞对溶瘤性水疱性口炎病毒敏感性的关键因素。
Autophagy Gene Activity May Act As a Key Factor for Sensitivity of Tumor Cells to Oncolytic Vesicular Stomatitis Virus.
作者信息
Nabizadeh Arezoo, Bamdad Taravat, Arefian Ehsan, Razavi Nikoo Seyed Hadi
机构信息
Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran.
Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, IR Iran.
出版信息
Iran J Cancer Prev. 2016 Feb 22;9(1):e3919. doi: 10.17795/ijcp-3919. eCollection 2016 Feb.
BACKGROUND
Beclin1 is an important, primary molecule for autophagy.
OBJECTIVES
It is suggested that the control of the autophagy path increases the sensitivity of tumor cells to VSV.
MATERIALS AND METHODS
In this study, the degree of Beclin1 gene expression in two cell lines, HeLa and A549, has been examined and the percentage of living cells subsequent infection with virus has been evaluated by MTT assay method.
RESULTS
The results showed that the degree of Beclin1 gene expression in HeLa cells in comparison with A549 cells has reduced, and the sensitivity of these cells to vesicular stomatits virus (VSV) oncolysis is more than A549.
CONCLUSIONS
It seems that by using some methods for reducing autophagy, it is possible to make tumor cells more sensitive to virotherapy and even other treatments.
背景
Beclin1是自噬的一个重要的主要分子。
目的
有人提出，控制自噬途径可增加肿瘤细胞对水泡性口炎病毒（VSV）的敏感性。
材料与方法
在本研究中，检测了HeLa和A549两种细胞系中Beclin1基因的表达程度，并通过MTT法评估了病毒感染后活细胞的百分比。
结果
结果显示，与A549细胞相比，HeLa细胞中Beclin1基因的表达程度降低，且这些细胞对水泡性口炎病毒（VSV）溶瘤的敏感性高于A549细胞。
结论
似乎通过使用一些降低自噬的方法，有可能使肿瘤细胞对病毒疗法甚至其他治疗更敏感。
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