Student Research Committee, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
Stem Cell Research Center, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
Asian Pac J Cancer Prev. 2021 Jan 1;22(1):249-255. doi: 10.31557/APJCP.2021.22.1.249.
Breast cancer is one of the most difficult malignancies to treat. Therapeutics is used to target and kill the cancer cells. Non-human oncolytic viruses have the ability to cause cell death directly to cancers. The objective here was to investigate the role of Vesicular Stomatitis Virus (VSV) Matrix (M) protein in autophagy in the breast cancer cell line.
Two different VSV wild type and mutant (M51R) M protein constructs were produced. Breast cancer cell line BT-20 was transfected by either wild type or mutant vectors. Transfection efficiency was measured using a fluorescent microscopy. Expression of VSV M protein was investigated at protein level. Cell cytotoxicity was measured using an MTT assay. The autophagy pathway was studied by Beclin-1 immunoassay. Data were statistically analyzed between different transfected groups.
It has been shown that the VSV M protein induced higher levels of Beclin-1 than the M51R mutant in the BT-20 cell line. Increased levels of Beclin-1 were also associated with VSV M cell-induced cytotoxicity.
It has been shown here that VSV wild type or mutant M proteins can cause autophagy-induced cell death by increasing Beclin-1 expression. This includes the possible role of VSV to be used as an oncolytic virus in breast cancer treatment.
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乳腺癌是最难治疗的恶性肿瘤之一。治疗方法用于靶向和杀死癌细胞。非人类溶瘤病毒有直接杀死癌细胞的能力。本研究旨在探讨水疱性口炎病毒（VSV）基质（M）蛋白在乳腺癌细胞系自噬中的作用。
制备了两种不同的 VSV 野生型和突变型（M51R）M 蛋白构建体。用野生型或突变型载体转染乳腺癌细胞系 BT-20。使用荧光显微镜测量转染效率。在蛋白质水平上研究了 VSV M 蛋白的表达。使用 MTT 测定法测量细胞细胞毒性。通过 Beclin-1 免疫测定法研究自噬途径。在不同转染组之间对数据进行了统计学分析。
结果表明，VSV M 蛋白在 BT-20 细胞系中诱导的 Beclin-1 水平高于 M51R 突变体。Beclin-1 水平的升高也与 VSV M 细胞诱导的细胞毒性相关。
本研究表明，VSV 野生型或突变型 M 蛋白可通过增加 Beclin-1 表达诱导自噬诱导的细胞死亡。这包括 VSV 用作乳腺癌治疗溶瘤病毒的可能作用。
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