Raclavský V, Hejtmánek M
Cesk Epidemiol Mikrobiol Imunol. 1989 May;38(3):161-6.
The authors describes a fluorescence microscopic method using fluorochrome Blankophor (Bayer) which binds to chitin of the cell walls of yeasts and filamentous fungi. The authors processed, using this method, 50 specimens of sputum and compared the results with those of cultivation examinations. In five instances, where cultivation was negative by the microscopic method large numbers of fungi were detected. The method is suitable for quantitative assessment of yeasts and other fungi in sputum and other clinical specimens (urine, irrigation of cavities etc.). The author discusses the interpretation of microscopic findings.
作者描述了一种使用荧光染料Blankophor（拜耳公司）的荧光显微镜方法，该染料可与酵母和丝状真菌细胞壁的几丁质结合。作者使用这种方法处理了50份痰液标本，并将结果与培养检查结果进行了比较。在5例培养结果为阴性的情况下，通过显微镜检查发现了大量真菌。该方法适用于对痰液和其他临床标本（尿液、腔隙灌洗液等）中的酵母和其他真菌进行定量评估。作者讨论了显微镜检查结果的解读。
