用荧光增白剂对真菌细胞壁进行染色：流式细胞仪分析
Staining of fungal cell walls with fluorescent brighteners: flow-cytometric analysis.
作者信息
Hejtmánek M, Dolezel J, Holubová I
机构信息
Department of Medical Biology, Faculty of Medicine, Palacký University, Olomouc, Czechoslovakia.
出版信息
Folia Microbiol (Praha). 1990;35(5):437-42. doi: 10.1007/BF02821413.
Spore walls of Backusella lamprospora (Mucorales) were stained with ten fluorescent brighteners (FB) and the intensity of their fluorescence was determined. The fluorescence was most intense with Uvitex 2B (100%), other brighteners yielding lower fluorescence intensities: Blankophor BA 267% and BA 200% about 75%, Rylux BSU about 50%, other Rylux agents 10-30%. The agents most suitable for microscopic diagnostics of human and animal mycoses are Uvitex 2B, Blankophor BA 267% and BA 200%, Rylux BSU, and also Rylux BS and PRS. The regulation of excessive fluorescence of fungal cells during microscopic observation is discussed. For the purposes of microscopic diagnosis of human and animal mycosis Uvitex 2B, Blankophor BA 267% and BA 200%, Rylux BSU and, possibly, Rylux BS and PRS are recommended.
用十种荧光增白剂（FB）对光亮后囊霉（毛霉目）的孢子壁进行染色，并测定其荧光强度。用Uvitex 2B染色时荧光最强（100%），其他增白剂产生的荧光强度较低：Blankophor BA 267%和BA 200%约为75%，Rylux BSU约为50%，其他Rylux试剂为10 - 30%。最适合用于人和动物真菌病显微镜诊断的试剂是Uvitex 2B、Blankophor BA 267%和BA 200%、Rylux BSU，还有Rylux BS和PRS。讨论了在显微镜观察过程中对真菌细胞过度荧光的调节。为了人和动物真菌病的显微镜诊断，推荐使用Uvitex 2B、Blankophor BA 267%和BA 200%、Rylux BSU，以及可能的Rylux BS和PRS。
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