德克萨斯州白尾鹿中伯氏疏螺旋体抗体的血清阳性率。

Seroprevalence of Borrelia burgdorferi antibodies in white-tailed deer from Texas.

作者信息

Adetunji Shakirat A, Krecek Rosina C, Castellanos Gabrielle, Morrill John C, Blue-McLendon Alice, Cook Walt E, Esteve-Gassent Maria D

机构信息

Department of Veterinary Pathobiology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA.

Department of Veterinary Pathobiology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA; Texas A&M One Health Initiative, Office of the Dean, Texas A&M University, College Station, TX 77843, USA; Department of Zoology, University of Johannesburg, Auckland Park, Johannesburg 2006, South Africa.

出版信息

Int J Parasitol Parasites Wildl. 2016 Jun 13;5(2):168-74. doi: 10.1016/j.ijppaw.2016.06.002. eCollection 2016 Aug.

DOI:10.1016/j.ijppaw.2016.06.002

PMID:27366674

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4919802/

Abstract

Lyme Disease is caused by the bacterial pathogen Borrelia burgdorferi, and is transmitted by the tick-vector Ixodes scapularis. It is the most prevalent arthropod-borne disease in the United States. To determine the seroprevalence of B. burgdorferi antibodies in white-tailed deer (Odocoileus virginianus) from Texas, we analyzed serum samples (n = 1493) collected during the 2001-2015 hunting seasons, using indirect ELISA. Samples with higher sero-reactivity (0.803 and above) than the negative control group (0.662) were further tested using a more specific standardized western immunoblot assay to rule out false positives. Using ELISA, 4.7% of the samples were sero-reactive against B. burgdorferi, and these originated in two eco-regions in Texas (Edwards Plateau and South Texas Plains). However, only 0.5% of the total samples were sero-reactive by standardized western immunoblot assay. Additionally, both ELISA and standardized western immunoblot assay results correlated with an increased incidence in human Lyme Disease cases reported in Texas. This is the first longitudinal study to demonstrate fluctuation in sero-reactivity of white-tailed deer to B. burgdorferi sensu stricto antigens in southern United States. Future ecological and geographical studies are needed to assess the environmental factors governing the prevalence of Lyme Disease in non-endemic areas of the southern United States.

摘要

莱姆病由细菌病原体伯氏疏螺旋体引起，通过蜱虫媒介肩突硬蜱传播。它是美国最普遍的节肢动物传播疾病。为了确定得克萨斯州白尾鹿（弗吉尼亚鹿）体内伯氏疏螺旋体抗体的血清阳性率，我们使用间接酶联免疫吸附测定法（ELISA）分析了在2001年至2015年狩猎季节采集的血清样本（n = 1493）。血清反应性高于阴性对照组（0.662）的样本（0.803及以上），进一步使用更特异的标准化免疫印迹法进行检测，以排除假阳性。使用ELISA检测时，4.7%的样本对伯氏疏螺旋体呈血清反应性，这些样本来自得克萨斯州的两个生态区（爱德华兹高原和南得克萨斯平原）。然而，通过标准化免疫印迹法检测，只有0.5%的总样本呈血清反应性。此外，ELISA和标准化免疫印迹法的检测结果均与得克萨斯州报告的人类莱姆病病例发病率增加相关。这是第一项证明美国南部白尾鹿对狭义伯氏疏螺旋体抗原的血清反应性存在波动的纵向研究。未来需要开展生态和地理研究，以评估影响美国南部非疫区莱姆病患病率的环境因素。