Bharmoria Arti, Vaish Vipin Behari, Chaurasia Ashish, Tahlan A K
National Influenza Surveillance Centre, Central Research Institute, Kasauli, HP India.
Virusdisease. 2016 Jun;27(2):130-5. doi: 10.1007/s13337-016-0312-1. Epub 2016 Apr 7.
The present study offers the status of ongoing surveillance influenza at National Influenza Surveillance Centre (NISC), Central Research Institute (CRI), Kasauli for the emergence and consistency of influenza strains for current as well as over a period of three decades in Solan, Himachal Pradesh. From 1980 to 2015, various clinical centers of Himachal reported counts of influenza-like illness (fever including cough or sore throat). During these years, the respiratory specimens of 6581 patients showing influenza-like illness were collected. The incidence of visits had been calculated for influenza-like illness using the size of the patient population, and the incidence attributable to influenza was extrapolated from the proportion of patients with positive tests. Up to 2008 the egg inoculation method was used for the isolation and detection of influenza strains but in 2009 a RT-PCR equipped, fabricated BSL-3 laboratory was implanted at CRI for the isolation and detection of influenza strains. The reagents, primers and probes were supplied by NCDC, Delhi. Since 1980, 319 influenza isolates has been identified and isolated at NISC. Among these 282 were isolated by egg propagation method while 37 were processed by RT-PCR. Influenza incidence varied with age groups and by season after the pandemic of 2009 influenza A, H1N1. High levels of influenza virus circulation, especially in young children, emphasize the need for additional efforts to increase the uptake of influenza vaccines and anti-virals.
本研究提供了位于喜马偕尔邦索兰市卡萨利中央研究所国家流感监测中心正在进行的流感监测状况，以了解当前以及过去三十年里流感毒株在该地的出现情况和一致性。从1980年到2015年，喜马偕尔邦的各个临床中心报告了流感样疾病（包括咳嗽或喉咙痛的发热）的病例数。在这些年里，收集了6581例表现出流感样疾病患者的呼吸道标本。利用患者群体规模计算了流感样疾病的就诊发病率，并从检测呈阳性的患者比例中推断出流感所致的发病率。直到2008年，一直采用鸡胚接种法来分离和检测流感毒株，但在2009年，中央研究所设立了一个配备实时荧光定量聚合酶链反应（RT-PCR）的生物安全3级实验室，用于分离和检测流感毒株。试剂、引物和探针由德里的国家疾病控制中心提供。自1980年以来，国家流感监测中心已鉴定并分离出319株流感病毒。其中282株通过鸡胚传代法分离，37株通过RT-PCR法处理。2009年甲型H1N1流感大流行后，流感发病率随年龄组和季节而变化。流感病毒的高传播水平，尤其是在幼儿中，凸显了加大力度提高流感疫苗和抗病毒药物接种率的必要性。
