Ataci Nese, Arsu Nergis
Yildiz Technical University, Chemistry Department, Davutpasa Campus, 34220 Esenler, Istanbul, Turkey.
Yildiz Technical University, Chemistry Department, Davutpasa Campus, 34220 Esenler, Istanbul, Turkey.
Spectrochim Acta A Mol Biomol Spectrosc. 2016 Dec 5;169:128-33. doi: 10.1016/j.saa.2016.06.032. Epub 2016 Jun 22.
In this study, a thioxanthone derivative named 2-(9-oxo-9H-thioxanthen-2ylamino) acetic acid (TX-NHCH2COOH) was used to investigate small molecule and DNA binding interactions. Absorption and fluorescence emission spectroscopy were used and melting studies were used to explain the binding mode of TXNHCH2COOH-DNA. Intrinsic binding constant Kb TXNHCH2COOH was found 6×10(5)M(-1)from UV-Vis absorption spectroscopy. Fluorescence emmision intensity increased by adding ct-DNA to the TXNHCH2COOH and KI quenching experiments resulted with low Ksv value. Additionally, 3.7°C increase for Tm was observed. The observed quenching of EB and ct-DNA complex and increase viscosity values of ct-DNA by addition of TXNHCH2COOH was determined. All those results indicate that TXNHCH2COOH can intercalate into DNA base pairs. Fluorescence microscopy helped to display imaging of the TXNHCH2COOH-DNA solution.
在本研究中，一种名为2-(9-氧代-9H-噻吨-2-基氨基)乙酸（TX-NHCH2COOH）的噻吨酮衍生物被用于研究小分子与DNA的结合相互作用。采用吸收光谱和荧光发射光谱，并通过熔解研究来解释TXNHCH2COOH与DNA的结合模式。通过紫外可见吸收光谱测得TXNHCH2COOH的固有结合常数Kb为6×10(5)M(-1)。向TXNHCH2COOH中加入ct-DNA后荧光发射强度增加，而KI猝灭实验得到的Ksv值较低。此外，观察到熔解温度（Tm）升高了3.7°C。测定了TXNHCH2COOH对EB与ct-DNA复合物的猝灭作用以及加入TXNHCH2COOH后ct-DNA粘度值的增加。所有这些结果表明TXNHCH2COOH可以插入到DNA碱基对中。荧光显微镜有助于展示TXNHCH2COOH-DNA溶液的成像。
