Tao Xian-Yao, Wang Long, Gao Wei-Yang, Ding Jian, Feng Xiao-Liang, Zhou Zong-Wei, Yang Liang-Hui
Department of Hand and Plastic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, The Second Clinical Medical College of Wenzhou Medical University, Wenzhou, China.
J Reconstr Microsurg. 2016 Nov;32(9):643-649. doi: 10.1055/s-0036-1584808. Epub 2016 Jul 1.
Inducible nitric oxide synthase (iNOS) plays an important role in vasodilation, angiogenesis, and ischemia-reperfusion injury. We investigated the effects of iNOS on the survival and choke vessels of multiterritory perforator flaps in rats. In this study, 84 rats were divided into two groups of 42 rats each and subjected to multiterritory perforator flap operations. Rats in group A received daily intraperitoneal doses of 100 mg per kg of aminoguanidine (AG) and rats in group B received daily intraperitoneal injections of the same volume of saline solution. On postoperative day 7, the surviving flap area was calculated as a percentage of the total flap dimensions using DP2-BSW software. The diameter and density of microvessels in the second choke zone of the flap were calculated from histology studies. The nitric oxide (NO) content was measured using NO concentration assay kits, and the levels of vascular endothelial growth factor (VEGF) and iNOS were assessed using western blotting. Superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were measured using test kits. Laser Doppler imaging was used to evaluate flap perfusion in the second choke zone for 7 days after surgery. The flap survival area, diameter and density of microvessels, iNOS and VEGF levels, NO content, blood perfusion, and MDA content were significantly higher in the control group compared with the AG group, whereas SOD activity was significantly lower in the control group. iNOS has a beneficial effect on the survival of multiterritory perforator flaps.
诱导型一氧化氮合酶（iNOS）在血管舒张、血管生成和缺血再灌注损伤中起重要作用。我们研究了iNOS对大鼠多区域穿支皮瓣存活及绞窄血管的影响。
在本研究中，84只大鼠被分为两组，每组42只，接受多区域穿支皮瓣手术。A组大鼠每天腹腔注射100mg/kg氨基胍（AG），B组大鼠每天腹腔注射相同体积的生理盐水。术后第7天，使用DP2-BSW软件计算存活皮瓣面积占皮瓣总面积的百分比。通过组织学研究计算皮瓣第二绞窄区微血管的直径和密度。使用一氧化氮浓度检测试剂盒测量一氧化氮（NO）含量，通过蛋白质免疫印迹法评估血管内皮生长因子（VEGF）和iNOS的水平。使用试剂盒测量超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量。术后7天使用激光多普勒成像评估皮瓣第二绞窄区的灌注情况。
与AG组相比，对照组的皮瓣存活面积、微血管直径和密度、iNOS和VEGF水平以及NO含量、血液灌注和MDA含量显著更高，而对照组的SOD活性显著更低。
iNOS对多区域穿支皮瓣的存活具有有益作用。
