Bazgir Fatemeh, Karimi Rouzbahani Arian, Birjandi Mehdi, Chehelcheraghi Farzaneh
Student Research Committee, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Nutritional Health Research Center, School of Health and Nutrition, Lorestan University of Medical Sciences, Khorramabad, Iran.
SAGE Open Med. 2024 Sep 6;12:20503121241276278. doi: 10.1177/20503121241276278. eCollection 2024.
Perforator flaps have recently been used in the field of plastic surgery. Skin defects can be reconstructed to reach functional and cosmetic goals. With the development of reconstructive approaches, utilizing stem cells is a hopeful approach to enhance wound healing and tissue recovery. In this study, we assessed the effect of bone marrow mesenchymal stem cells on the perforator flap's survival in rats.
Perforator flaps (2.5 × 11 cm) were transplanted into rats and focused on the thoracodorsal, intercostal, and deep circumflex arteries, which were randomly divided into three groups: control, saline, and bone marrow mesenchymal stem cells (7 × 10 ml). Seven days after the surgery, tissue edema, inflammation, and discharge were observed and photographed. Histological analyses were performed to determine flap survival. Hematoxylin and eosin staining was performed to assess levels of microvascular density determined in skin flaps.
Rats in the bone marrow mesenchymal stem cells group exhibited higher average flap survival area, and higher microvascular density levels at the dynamical regions of the flaps compared with the other two groups. Subdermal injection of bone marrow mesenchymal stem cells significantly increased ischemic perforator flap survival due to stimulated neovascularization in rats.
Our findings suggest the potential usefulness of bone marrow mesenchymal stem cells in preventing skin flap perforator tissue necrosis.
穿支皮瓣最近已应用于整形手术领域。皮肤缺损可通过重建达到功能和美观的目的。随着重建方法的发展，利用干细胞是促进伤口愈合和组织恢复的一种有前景的方法。在本研究中，我们评估了骨髓间充质干细胞对大鼠穿支皮瓣存活的影响。
将穿支皮瓣（2.5×11厘米）移植到大鼠体内，皮瓣集中于胸背动脉、肋间动脉和旋髂深动脉，随机分为三组：对照组、生理盐水组和骨髓间充质干细胞组（7×10毫升）。术后7天，观察并拍摄组织水肿、炎症和分泌物情况。进行组织学分析以确定皮瓣存活情况。采用苏木精-伊红染色评估皮瓣中微血管密度水平。
与其他两组相比，骨髓间充质干细胞组大鼠的皮瓣平均存活面积更大，皮瓣动态区域的微血管密度水平更高。皮下注射骨髓间充质干细胞可通过刺激大鼠新生血管形成，显著提高缺血穿支皮瓣的存活率。
我们的研究结果表明骨髓间充质干细胞在预防皮瓣穿支组织坏死方面具有潜在作用。
