Moreno Patricia, Garcia-Rosado Esther, Borrego Juan J, Alonso M Carmen
Universidad de Málaga, Departamento de Microbiología, Facultad de Ciencias, 29071 Málaga, Spain.
Universidad de Málaga, Departamento de Microbiología, Facultad de Ciencias, 29071 Málaga, Spain.
Fish Shellfish Immunol. 2016 Aug;55:642-6. doi: 10.1016/j.fsi.2016.06.043. Epub 2016 Jun 29.
Fish interferons are cytokines involved in its resistance to viral infections by inducing the transcription of several interferon-induced genes, such as isg15. The aim of the present study was the genetic characterization of the European sea bass isg15 gene, describing the regulatory motifs found in its sequence. In addition, an in vivo analysis of transcription in response to betanodavirus (RGNNV genotype) and poly I:C has been performed. The analysis of the resulting sequences showed that sea bass isg15 gene is composed of two exons and a single 276-bp intron located at the 5'-UTR region. The full length cDNA is 1143-bp, including a 102-bp 5'-UTR region, a 474-bp ORF, and a 291-bp 3'-UTR region. Several mRNA-regulatory elements, including three unusual ATTTA instability motifs in the intron, and four ATTTA motifs along with a cytoplasmic polyadenylation element in the 3'-UTR region, have been found in this sequence. The in vivo analyses revealed a similar kinetics and level of transcription in fish brain and head kidney after poly I:C inoculation; however, the induction caused by RGNNV started earlier in brain, where the upregulation of isg15 gene transcription was high. The present study contributes to further characterize the European sea bass IFN I response against RGNNV infections.
鱼类干扰素是一类细胞因子，通过诱导多种干扰素诱导基因（如isg15）的转录参与其对病毒感染的抗性。本研究的目的是对欧洲海鲈isg15基因进行遗传特征分析，描述其序列中发现的调控基序。此外，还进行了体内分析，以研究其对贝塔诺病毒（RGNNV基因型）和聚肌胞苷酸（poly I:C）的转录反应。对所得序列的分析表明，海鲈isg15基因由两个外显子和一个位于5'-UTR区域的276 bp内含子组成。全长cDNA为1143 bp，包括一个102 bp的5'-UTR区域、一个474 bp的开放阅读框（ORF）和一个291 bp 的3'-UTR区域。在该序列中发现了几个mRNA调控元件，包括内含子中的三个不寻常的ATTTA不稳定基序，以及3'-UTR区域中的四个ATTTA基序和一个细胞质聚腺苷酸化元件。体内分析显示，聚肌胞苷酸接种后，鱼脑和头肾中的转录动力学和水平相似；然而，RGNNV引起的诱导在脑中开始得更早，其中isg15基因转录的上调程度很高。本研究有助于进一步表征欧洲海鲈针对RGNNV感染的I型干扰素反应。
