Sugai Kyoko, Setsuko Suzuki
Laboratory of Wildlife Ecology, Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba, Ibaraki, 305-8687, Japan.
Laboratory of Ecological Genetics, Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba, Ibaraki, 305-8687, Japan.
BMC Res Notes. 2016 Jul 2;9:332. doi: 10.1186/s13104-016-2137-9.
Distylium lepidotum is a small tree endemic to the Ogasawara Islands located in the northwestern Pacific Ocean. This species is a sole food for an endemic locust, Boninoxya anijimensis. Here, we developed microsatellite markers to investigate genetic diversity and genetic structure and to avoid a genetic disturbance after transplantation to restore the Ogasawara Islands ecosystem.
Microsatellite markers with perfect dinucleotide repeats were developed using the next-generation sequencing Illumina MiSeq Desktop Sequencer. Thirty-two primer pairs were characterized in two D. lepidotum populations on Chichijima and Hahajima Islands of the Ogasawara Islands. The number of alleles for the markers ranged from three to 23 per locus in the two populations. Expected heterozygosity per locus in each population ranged from 0.156 to 0.940 and 0.368 to 0.845, respectively.
These microsatellite markers will be useful for future population genetics studies of D. lepidotum and provide a basis for conservation management of the Ogasawara Islands.
鳞毛蚊母树是一种生长于太平洋西北部小笠原群岛的小型树木。该物种是当地特有蝗虫——阿尼吉门斯氏邦尼蝗的唯一食物来源。在此，我们开发了微卫星标记，以研究其遗传多样性和遗传结构，并避免移植后出现基因干扰，从而恢复小笠原群岛的生态系统。
利用下一代测序技术Illumina MiSeq桌面测序仪开发了具有完美二核苷酸重复序列的微卫星标记。在小笠原群岛父岛和母岛的两个鳞毛蚊母树种群中对32对引物进行了特征分析。两个种群中每个标记的等位基因数量范围为每个位点3至23个。每个种群中每个位点的预期杂合度分别为0.156至0.94以及0.368至0.845。
这些微卫星标记将有助于未来对鳞毛蚊母树进行种群遗传学研究，并为小笠原群岛的保护管理提供依据。
