Cortés Jimena, Alvarez Claudio, Santana Paula, Torres Elisa, Mercado Luis
Grupo de Marcadores Inmunológicos, Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.
Grupo de Marcadores Inmunológicos, Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile; Programa de Doctorado en Biotecnología, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile; Universidad Técnica Federico Santa María, Valparaíso, Chile.
Dev Comp Immunol. 2016 Dec;65:73-78. doi: 10.1016/j.dci.2016.06.020. Epub 2016 Jun 29.
The role of enzymes as active antimicrobial agents of the innate immunity in teleost fish is proposed in diverse works. Secretion of Indoleamine 2,3-dioxygenase (IDO) has been described in higher vertebrates; it degrades l-tryptophan in extracellular environments associated mainly with mucosal organs. The effect of IDO on decreasing amino acid concentration may inhibit the growth of potential pathogens. In fish the study of this molecule is still. Here we report the identification of an Onchorhyncus mykiss IDO homologue (OmIDO). IDO was cloned, sequenced, and the primary structure shows conservation of key functional sites. The constitutive expression is altered when the fish is challenged with LPS as a pathogen-associated molecular pattern (PAMPs). Up-regulation of IDO was shown preferentially in the fish's mucosal cells. In order to obtain evidence of a possible regulation mechanism, an in vitro cell model was used for to show that OmIDO is induced by rIFN. These study has identified a Indoleamine 2,3-dyoxigenase in O. mykiss will contribute to expands our knowledge of the function this protein in fish immune response. These findings allow to propose the use of OmIDO as a molecular indicator of strength of the animal's immune response and wellbeing.
在不同的研究中提出了酶作为硬骨鱼先天免疫的活性抗菌剂的作用。吲哚胺2,3-双加氧酶（IDO）的分泌已在高等脊椎动物中得到描述；它在主要与粘膜器官相关的细胞外环境中降解L-色氨酸。IDO对降低氨基酸浓度的作用可能会抑制潜在病原体的生长。在鱼类中，对这种分子的研究仍在进行中。在这里，我们报告了虹鳟鱼IDO同源物（OmIDO）的鉴定。对IDO进行了克隆、测序，其一级结构显示关键功能位点具有保守性。当鱼受到作为病原体相关分子模式（PAMP）的LPS攻击时，其组成型表达会发生改变。IDO的上调优先出现在鱼的粘膜细胞中。为了获得可能的调控机制的证据，使用体外细胞模型表明OmIDO由重组干扰素（rIFN）诱导。这些研究已经在虹鳟鱼中鉴定出一种吲哚胺2,3-双加氧酶，这将有助于扩展我们对该蛋白在鱼类免疫反应中功能的认识。这些发现使得我们能够提出将OmIDO用作动物免疫反应强度和健康状况的分子指标。
