原位杂交揭示人类重复DNA的细胞质定位
Cytoplasmic localization of human repetitive DNA revealed by in situ hybridization.
作者信息
Kiyama R, Oishi M, Kanda N
机构信息
Institute of Applied Microbiology, University of Tokyo, Japan.
出版信息
Exp Cell Res. 1989 Jul;183(1):239-44. doi: 10.1016/0014-4827(89)90433-3.
Previously, we showed that a human repetitive DNA sequence (Sau3A family) belonging to a satellite DNA is unstable and constantly excised from the chromosomes (R. Kiyama, H. Matsui, and M. Oishi, 1986, Proc. Natl. Acad. Sci. USA 83, 4665). The unusual property of the repetitive DNA, along with another repetitive DNA (Alu sequence), was further investigated by in situ hybridization in several different human cells including HeLa, bone marrow, and peripheral blood cells. We found that the excised repetitive DNA sequences are localized not only in nuclei, but also in cytoplasm. These results have confirmed the instability of these DNA sequences in the chromosomes and further suggest that the alpha satellite DNA and the Alu sequence which were excised from the chromosomes are released from nuclei to cytoplasm.
此前，我们发现属于卫星DNA的一个人类重复DNA序列（Sau3A家族）不稳定，会不断从染色体上切除（R. 木山、H. 松井和M. 大石，1986年，《美国国家科学院院刊》83卷，4665页）。通过在包括HeLa细胞、骨髓细胞和外周血细胞在内的几种不同人类细胞中进行原位杂交，对该重复DNA以及另一个重复DNA（Alu序列）的异常特性进行了进一步研究。我们发现，切除的重复DNA序列不仅定位于细胞核中，也定位于细胞质中。这些结果证实了这些DNA序列在染色体中的不稳定性，并进一步表明从染色体上切除的α卫星DNA和Alu序列从细胞核释放到了细胞质中。
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