Carrino D A, Kujawa M J, Lennon D P, Caplan A I
Department of Biology, Case Western Reserve University, Cleveland, Ohio 44106.
Exp Cell Res. 1989 Jul;183(1):62-71. doi: 10.1016/0014-4827(89)90418-7.
Chick high-density culture chondrocytes synthesize cartilage-specific proteoglycans with much structural similarity to the proteoglycans made by cartilage in vivo. Such cultures can be maintained in a defined medium formulated in this laboratory in which chondrogenesis occurs without the addition of serum. The proteoglycans synthesized by the chondrocytes in the presence of defined medium are of a cartilage-specific structure but differ in some aspects from the proteoglycans made in serum-containing medium. While their buoyant density, ability to aggregate with hyaluronic acid, and keratan sulfate chain size are unchanged, the proteoglycans synthesized in defined medium have altered chondroitin sulfate chains. This chondroitin sulfate is of significantly larger size and has a different sulfation pattern relative to that produced in serum-containing medium. The larger size of the chondroitin sulfate results in a larger monomer size of the defined medium proteoglycans. These differences have implications about the regulation of the structure of chondroitin sulfate proteoglycans.
鸡高密度培养软骨细胞合成的软骨特异性蛋白聚糖在结构上与体内软骨产生的蛋白聚糖非常相似。这种培养物可以在本实验室配制的特定培养基中维持，在不添加血清的情况下发生软骨形成。在特定培养基存在下软骨细胞合成的蛋白聚糖具有软骨特异性结构，但在某些方面与含血清培养基中产生的蛋白聚糖不同。虽然它们的浮力密度、与透明质酸聚集的能力以及硫酸角质素链大小没有变化，但在特定培养基中合成的蛋白聚糖的硫酸软骨素链发生了改变。这种硫酸软骨素的大小明显更大，并且相对于含血清培养基中产生的硫酸软骨素具有不同的硫酸化模式。硫酸软骨素较大的尺寸导致特定培养基蛋白聚糖的单体尺寸更大。这些差异对硫酸软骨素蛋白聚糖结构的调节具有影响。
