Miñana M D, Felipo V, Wallace R, Grisolía S
Instituto de Investigaciones Citológicas de la Caja de Ahorros de Valencia, Centro Asociado de CSIC, Spain.
FEBS Lett. 1989 Jun 5;249(2):261-3. doi: 10.1016/0014-5793(89)80636-2.
We have developed an animal model of hyperammonemia consisting of feeding rats a diet containing 20% (w/w) ammonium acetate. Ingestion of this diet markedly affects carcass composition, with a 46% reduction in lipid content. The ammonium diet alters levels of several key compounds involved in lipid metabolism. Long-chain acylcarnitine is increased in liver by approx. 60% while free carnitine and acetylcarnitine are unaffected. The hepatic content of acetyl-CoA increases by approx. 50%. The level of ketone bodies in blood increases by 32% but remains unchanged in liver. Our data indicate that hyperammonemia alters lipid metabolism and results in a significant decrease in body lipid content.
我们已经建立了一种高氨血症动物模型，方法是给大鼠喂食含20%（重量/重量）醋酸铵的饮食。摄入这种饮食会显著影响胴体组成，脂质含量降低46%。含铵饮食会改变参与脂质代谢的几种关键化合物的水平。肝脏中长链酰基肉碱增加约60%，而游离肉碱和乙酰肉碱不受影响。肝脏中乙酰辅酶A的含量增加约50%。血液中酮体水平增加32%，但肝脏中保持不变。我们的数据表明，高氨血症会改变脂质代谢并导致机体脂质含量显著降低。
