Fei Yao, Xiong Yajie, Zhao Yifan, Wang Wenjuan, Han Meilin, Wang Long, Tan Caihong, Liang Zhongqin
Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou, China.
Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou, China; Department of Pharmacy, Affiliated Hospital of Jiangsu University, Zhenjiang, China.
Brain Res. 2016 Sep 1;1646:580-588. doi: 10.1016/j.brainres.2016.06.046. Epub 2016 Jul 1.
Curcumin can be used to prevent and treat cancer. However, its exact underlying molecular mechanisms remain poorly understood. Cathepsin L, a lysosomal cysteine protease, is overexpressed in several cancer types. This study aimed to determine the role of cathepsin L in curcumin-mediated inhibition of growth, migration, and invasion of glioma cells. Results revealed that the activity of cathepsin L was enhanced in curcumin-treated glioma cells. Cathepsin L knockdown induced by RNA interference significantly promoted curcumin-induced cytotoxicity, apoptosis, and cell cycle arrest. The knockdown also inhibited the migration and invasion of glioma cells. Our results suggested that the inhibition of cathepsin L can enhance the sensitivity of glioma cells to curcumin. Therefore, cathepsin L may be a new target to enhance the efficacy of curcumin against cancers.
姜黄素可用于预防和治疗癌症。然而，其确切的潜在分子机制仍知之甚少。组织蛋白酶L是一种溶酶体半胱氨酸蛋白酶，在几种癌症类型中均有过表达。本研究旨在确定组织蛋白酶L在姜黄素介导的抑制胶质瘤细胞生长、迁移和侵袭中的作用。结果显示，在姜黄素处理的胶质瘤细胞中，组织蛋白酶L的活性增强。RNA干扰诱导的组织蛋白酶L敲低显著促进了姜黄素诱导的细胞毒性、细胞凋亡和细胞周期停滞。敲低还抑制了胶质瘤细胞的迁移和侵袭。我们的结果表明，抑制组织蛋白酶L可增强胶质瘤细胞对姜黄素的敏感性。因此，组织蛋白酶L可能是增强姜黄素抗癌疗效的新靶点。
