Dang Bingjun, Xu Yan, Mao Daqing, Luo Yi
School of Environmental Science and Engineering, Tianjin University, Tianjin, China; Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, Tianjin, China.
School of Environmental Science and Engineering, Tianjin University, Tianjin, China.
Gene. 2016 Oct 10;591(1):74-79. doi: 10.1016/j.gene.2016.06.060. Epub 2016 Jul 1.
Antibiotic resistance is a serious problem in health care and is of widespread public concern. Conjugative plasmids are the most important vectors in the dissemination of antibiotic resistance genes. In this study, we determined the complete sequence of plasmid pNA6, a plasmid which was isolated from the sediments of Haihe River. This plasmid confers reduced susceptibility to ampicillin, erythromycin and sulfamethoxazole. The complete sequence of plasmid pNA6 was 52,210bp in length with an average G+C content of 52.70%. Plasmid pNA6 belongs to the IncU group by sequence queries against the GenBank database. This plasmid has a typical IncU backbone and shows the highest similarities with plasmid RA3 and plasmid pFBAOT6. Plasmid pNA6 carries a class 1 integron consisting of aacA4, ereA and dfrA1 genes. Moreover, plasmid pNA6 also harbors a blaTEM-1-containing complex structure which inserted into the replication region and maintenance region. This insertion site has never been found on other IncU plasmids. The sequencing of plasmid pNA6 will add new sequence information to IncU family plasmids and enhance our understanding of the plasticity of IncU family plasmids.
抗生素耐药性是医疗保健领域的一个严重问题，受到广泛的公众关注。接合质粒是抗生素耐药基因传播中最重要的载体。在本研究中，我们测定了从海河沉积物中分离出的质粒pNA6的完整序列。该质粒对氨苄青霉素、红霉素和磺胺甲恶唑的敏感性降低。质粒pNA6的完整序列长度为52210bp，平均G+C含量为52.70%。通过对GenBank数据库进行序列查询，质粒pNA6属于IncU组。该质粒具有典型的IncU主干，与质粒RA3和质粒pFBAOT6的相似性最高。质粒pNA6携带一个由aacA4、ereA和dfrA1基因组成的1类整合子。此外，质粒pNA6还含有一个插入复制区和维持区的含blaTEM-1的复杂结构。该插入位点在其他IncU质粒上从未发现过。质粒pNA6的测序将为IncU家族质粒增添新的序列信息，并增强我们对IncU家族质粒可塑性的理解。
