Wu Qian, Liu Tingting, Zhu Liying, Huang He, Jiang Ling
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 210019, People's Republic of China.
Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University, Nanjing, 210019, People's Republic of China.
J Ind Microbiol Biotechnol. 2017 Aug;44(8):1245-1260. doi: 10.1007/s10295-017-1956-6. Epub 2017 May 23.
Genetic research enables the evolution of novel biochemical reactions for the production of valuable chemicals from environmentally-friendly raw materials. However, the choice of appropriate microorganisms to support these reactions, which must have strong robustness and be capable of a significant product output, is a major difficulty. In the present study, the complete genome of the Clostridium tyrobutyricum strain CCTCC W428, a hydrogen- and butyric acid-producing bacterium with increased oxidative tolerance was analyzed. A total length of 3,011,209 bp of the C. tyrobutyricum genome with a GC content of 31.04% was assembled, and 3038 genes were discovered. Furthermore, a comparative clustering of proteins from C. tyrobutyricum CCTCC W428, C. acetobutylicum ATCC 824, and C. butyricum KNU-L09 was conducted. The results of genomic analysis indicate that butyric acid is produced by CCTCC W428 from butyryl-CoA through acetate reassimilation via CoA transferase, instead of the well-established phosphotransbutyrylase-butyrate kinase pathway. In addition, we identified ten proteins putatively involved in hydrogen production and 21 proteins associated with CRISPR systems, together with 358 ORFs related to ABC transporters and transcriptional regulators. Enzymes, such as oxidoreductases, HNH endonucleases, and catalase, were also found in this species. The genome sequence illustrates that C. tyrobutyricum has several desirable traits, and is expected to be suitable as a platform for the high-level production of bulk chemicals as well as bioenergy.
基因研究有助于开发新的生化反应，以便从环境友好型原料生产有价值的化学品。然而，选择合适的微生物来支持这些反应存在很大困难，因为这些微生物必须具有很强的稳健性，并且能够大量产出产品。在本研究中，分析了酪丁酸梭菌菌株CCTCC W428的完整基因组，该菌株是一种具有增强氧化耐受性的产氢和丁酸细菌。组装得到了酪丁酸梭菌基因组全长3,011,209 bp，GC含量为31.04%，并发现了3038个基因。此外，还对酪丁酸梭菌CCTCC W428、丙酮丁醇梭菌ATCC 824和丁酸梭菌KNU-L09的蛋白质进行了比较聚类。基因组分析结果表明，CCTCC W428通过辅酶A转移酶将乙酸重新同化，从丁酰辅酶A产生丁酸，而不是通过成熟的磷酸转丁酰酶-丁酸激酶途径。此外，我们鉴定了十种可能参与产氢的蛋白质和21种与CRISPR系统相关的蛋白质，以及358个与ABC转运蛋白和转录调节因子相关的开放阅读框。在该物种中还发现了氧化还原酶、HNH核酸内切酶和过氧化氢酶等酶。基因组序列表明，酪丁酸梭菌具有几个理想的特性，有望成为大量化学品以及生物能源的高产平台。
