Lo Kimberly L, Rosenkrantz Wayne S
Animal Dermatology Clinic, 2965 Edinger Avenue, Tustin, CA, 92780, USA.
Vet Dermatol. 2016 Aug;27(4):279-e67. doi: 10.1111/vde.12297.
Canine bacterial and Malassezia paronychia are common secondary complications of atopic dermatitis and adverse food reactions.
HYPOTHESIS/OBJECTIVES: The aim of this study was to compare three different sampling methods for claw fold cytology and to evaluate the numbers of bacteria, Malassezia yeast and inflammatory cells.
Sixty client-owned dogs were classified into three groups: (A) normal dogs; (B) allergic dogs with no clinical evidence of claw disease (brown staining, erythema, swelling, crusts or exudates); and (C) allergic dogs with clinical paronychia.
A prospective, blinded, split-plot study design was used. Claw folds from each dog were sampled using either a toothpick, tape preparation or direct impression smear. Slides were evaluated by two investigators for inflammatory cells, nuclear streaming, debris, corneocytes, yeast, intracellular (IC) cocci, extracellular (EC) cocci, IC rods and EC rods. For each parameter, data were compared between groups and between methods. Inter-reader agreements were calculated.
Group C had significantly higher values of EC cocci and corneocytes than Groups A or B. Although Malassezia organisms were more prevalent in allergic dogs than normal dogs, the counts were not significantly different. There were significantly higher numbers of Malassezia organisms (P = 0.0016) and EC cocci (P = 0.0106) retrieved from samples collected with a toothpick compared to other methods. Tape preparations were associated with significantly more debris and corneocytes (both P < 0.0001) and impression smears with significantly more nuclear streaming (P = 0.0468).
Sample collection using a toothpick optimizes the value of cytological results when sampling allergic dogs with clinical paronychia.
犬细菌性甲沟炎和马拉色菌性甲沟炎是特应性皮炎和食物不良反应常见的继发性并发症。
假设/目的：本研究旨在比较三种不同的爪褶细胞学采样方法，并评估细菌、马拉色菌酵母和炎性细胞的数量。
60只客户拥有的犬分为三组：（A）正常犬；（B）无爪病临床证据（棕色染色、红斑、肿胀、结痂或渗出物）的过敏性犬；（C）患有临床甲沟炎的过敏性犬。
采用前瞻性、盲法、裂区研究设计。使用牙签、胶带制片或直接印片法对每只犬的爪褶进行采样。两名研究人员对玻片进行炎性细胞、核流、碎屑、角质形成细胞、酵母、细胞内（IC）球菌、细胞外（EC）球菌、IC杆菌和EC杆菌的评估。对于每个参数，比较组间和方法间的数据。计算阅片者间的一致性。
C组的EC球菌和角质形成细胞值显著高于A组或B组。虽然马拉色菌在过敏性犬中比正常犬更普遍，但计数无显著差异。与其他方法相比，用牙签采集的样本中马拉色菌（P = 0.0016）和EC球菌（P = 0.0106）的数量显著更多。胶带制片与显著更多的碎屑和角质形成细胞相关（均P < 0.0001），而印片法与显著更多的核流相关（P = 0.0468）。
对患有临床甲沟炎的过敏性犬进行采样时，使用牙签采集样本可优化细胞学检查结果的价值。
