通过菌落免疫印迹法检测细菌细胞表面的肠杆菌共同抗原：其与脂多糖连接的影响。
Detection of enterobacterial common antigen on bacterial cell surfaces by colony-immunoblotting: effect of its linkage to lipopolysaccharide.
作者信息
Meier-Dieter U, Acker G, Mayer H
机构信息
Max-Planck-Institut für Immunbiologie, Freiburg, F.R.G.
出版信息
FEMS Microbiol Lett. 1989 May;50(1-2):215-9. doi: 10.1016/0378-1097(89)90489-8.
PMID:2737463
Abstract
A colony-immunoblotting procedure is described which allows a quick screening of high numbers of bacteria for their Enterobacterial Common Antigen phenotype. In this assay the intensity of reaction is dependent on the carrier to which ECA is linked and which anchors ECA in the outer membrane. Bacteria containing LPS-linked ECA react stronger in this and in other immunoassays than bacteria containing only the phospholipid-linked ECA.
摘要
本文描述了一种菌落免疫印迹法，该方法可快速筛选大量细菌的肠杆菌共同抗原表型。在该检测中，反应强度取决于与肠杆菌共同抗原（ECA）相连并将其锚定在外膜中的载体。与仅含有磷脂连接的ECA的细菌相比，含有脂多糖连接的ECA的细菌在该检测及其他免疫检测中反应更强。
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