肠出血性大肠杆菌O157:H7黏附特性及侵袭潜力的研究揭示了肠道大肠杆菌共有的一种新附着模式。
Investigation of enterohemorrhagic Escherichia coli O157:H7 adherence characteristics and invasion potential reveals a new attachment pattern shared by intestinal E. coli.
作者信息
McKee M L, O'Brien A D
机构信息
Department of Microbiology and Immunology, Uniformed Service University of the Health Sciences, F. Edward Hébert School of Medicine, Bethesda, Maryland 20814-4799, USA.
出版信息
Infect Immun. 1995 May;63(5):2070-4. doi: 10.1128/iai.63.5.2070-2074.1995.
Abstract
In this study, the interactions of enterohemorrhagic Escherichia coli (EHEC) O157 strains with human ileocecal (HCT-8) epithelial cells and HEp-2 cells were examined. EHEC adhered to, but did not invade, HCT-8 cells by the localized adherence mechanism and a heretofore unrecognized pattern which we called log jam. The log jam formation was (i) not observed on HEp-2 cells, (ii) independent of the EHEC eaeA gene required for localized adherence, and (iii) shared by pathogenic and nonpathogenic E. coli strains but not K-12 strains. The log jam phenotype may represent a basal means by which E. coli bacteria attach to the human intestine.
摘要
在本研究中，对肠出血性大肠杆菌（EHEC）O157菌株与人回盲部（HCT-8）上皮细胞及人喉表皮样癌细胞（HEp-2细胞）之间的相互作用进行了检测。EHEC通过局部黏附机制以及一种我们称之为“堵塞”的前所未有的模式黏附于HCT-8细胞，但并未侵入细胞。“堵塞”的形成有以下特点：（i）在HEp-2细胞上未观察到；（ii）独立于局部黏附所需的EHEC eaeA基因；（iii）致病性和非致病性大肠杆菌菌株均有此现象，但K-12菌株没有。“堵塞”表型可能代表大肠杆菌附着于人类肠道的一种基本方式。
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