Department of Soil, Plant and Food Science, University of Bari Aldo Moro, 70126, Bari, Italy.
Department of Soil, Plant and Food Science, University of Bari Aldo Moro, 70126, Bari, Italy.
Food Microbiol. 2016 Oct;59:176-89. doi: 10.1016/j.fm.2016.06.009. Epub 2016 Jun 10.
Strains of Leuconostoc mesenteroides were identified from raw prickly pear (Opuntia ficus-indica L.). Five autochthonous strains were selected based on the kinetics of growth and acidification on prickly pear fruit juice, and the capacity to synthesize exo-polysaccharides. All selected Leuc. mesenteroides strains showed an in vitro mucilage-degrading capability. A protocol for processing and storage of fermented prickly pear fruit puree (FP) was set up. Unstarted FP and chemically acidified FP were used as the controls. Starters grew and remained viable at elevated cell numbers during 21 days of storage at 4 °C. Contaminating Enterobacteriaceae and yeasts were found only in the controls. Viscosity and serum separation distinguished started FP compared to the controls. Colour parameters, browning index, sensory attributes, antimicrobial activity, vitamin C and betalains levels were positively affected by lactic acid fermentation. Increase of free radical scavenging activity in ethyl acetate soluble extract suggested an effect of selected strains on phenolic profiles. Started FP markedly inhibited the inflammatory status of Caco-2/TC7 cells, and also contributed to maintaining the integrity of tight junctions. Started FP scavenged the reactive oxygen species generated by H2O2 on Caco-2 cells. All selected strain variously affected the immunomodulatory activity towards anti- and pro-inflammatory cytokines.
从仙人掌（Opuntia ficus-indica L.）中分离出肠膜明串珠菌（Leuconostoc mesenteroides）菌株。根据在仙人掌果实汁中的生长和酸化动力学以及合成胞外多糖的能力，选择了 5 种本土菌株。所有选定的肠膜明串珠菌菌株均显示出体外黏液降解能力。建立了发酵仙人掌果实泥（FP）的加工和储存方案。未启动的 FP 和化学酸化的 FP 用作对照。在 4°C 下储存 21 天时，起始物在升高的细胞数量下生长并保持存活。仅在对照物中发现了肠杆菌科和酵母菌的污染。与对照物相比，启动的 FP 的粘度和血清分离得到了区分。粘度和血清分离区分了启动的 FP 与对照物。颜色参数、褐变指数、感官属性、抗菌活性、维生素 C 和甜菜红素水平均受到乳酸发酵的积极影响。乙酸乙酯可溶提取物中自由基清除活性的增加表明所选菌株对酚类物质图谱有影响。启动的 FP 显著抑制了 Caco-2/TC7 细胞的炎症状态，并且有助于维持紧密连接的完整性。启动的 FP 清除了 Caco-2 细胞中 H2O2 产生的活性氧。所选菌株对抗炎和促炎细胞因子的免疫调节活性有不同的影响。
