Sampaio Karoliny Brito, de Albuquerque Thatyane Mariano Rodrigues, Rodrigues Noádia Priscila Araújo, de Oliveira Maria Elieidy Gomes, de Souza Evandro Leite
Laboratory of Food Microbiology, Department of Nutrition, Health Sciences Center, Federal University of Paraíba, João Pessoa 58051-900, Paraíba, Brazil.
Laboratory of Bromatology, Department of Nutrition, Health Sciences Center, Federal University of Paraíba, João Pessoa 58051-900, Paraíba, Brazil.
Foods. 2021 Dec 1;10(12):2960. doi: 10.3390/foods10122960.
(A. Weber ex. K. Schum.) Bly. ex Rowl., popularly known as xique-xique, is a cactus from the Caatinga biome, which is rich in bioactive compounds but has not been previously studied as a source of lactic acid bacteria (LAB) with probiotic aptitudes. This study aimed to identify, characterize, and select LAB isolates with in vitro probiotic-related characteristics from xique-xique cladodes and fruit. Isolates with the most promising probiotic-related characteristics were evaluated regarding their in vitro technological properties and capability of surviving in chestnut milk, whey protein drink, and mate tea with mint during 21 days of refrigeration storage. Seventeen recovered isolates had typical characteristics of LAB. Six out of these seventeen LAB isolates passed the safety tests and were included in experiments to evaluate the in vitro probiotic-related characteristics. Based on the results of a principal component analysis, the isolates 69, 82, 98, and 108 had the best performances in experiments to evaluate the probiotic-related characteristics. In addition to showing good technological properties, the four selected LAB isolates had high viable counts (>7.3 log cfu/mL) and high sizes of physiologically active cell subpopulations in chestnut milk, whey protein drink, and mate tea during refrigeration storage. These four isolates were identified by 16S-rRNA sequencing as being or . The results indicate xique-xique as a source of potentially probiotic LAB isolates.
（A. 韦伯 原作者 K. 舒姆）布莱 原作者 罗尔，俗称西克西克，是一种来自卡廷加生物群落的仙人掌，富含生物活性化合物，但此前尚未作为具有益生菌特性的乳酸菌（LAB）来源进行研究。本研究旨在从西克西克的茎和果实中鉴定、表征并筛选出具有体外益生菌相关特性的LAB分离株。对具有最有前景的益生菌相关特性的分离株进行了体外技术特性评估，以及在冷藏储存21天期间在栗奶、乳清蛋白饮料和薄荷马黛茶中存活能力的评估。17株分离株具有LAB的典型特征。这17株LAB分离株中有6株通过了安全性测试，并被纳入评估体外益生菌相关特性的实验。基于主成分分析结果，分离株69、82、98和108在评估益生菌相关特性的实验中表现最佳。除了具有良好的技术特性外，这4株选定的LAB分离株在冷藏储存期间在栗奶、乳清蛋白饮料和薄荷马黛茶中具有高活菌数（>7.3 log cfu/mL）和高生理活性细胞亚群数量。通过16S - rRNA测序鉴定这4株分离株为 或 。结果表明西克西克是潜在益生菌LAB分离株的一个来源。
