吲哚美辛对人胎盘绒毛干动脉的影响。

Effects of indomethacin on human placental stem villous arteries.

作者信息

Allen J, Lauridsen V, Hansen V, Andersson K E, Forman A

机构信息

Department of Obstetrics and Gynecology, University of Aarhus, Denmark.

出版信息

Gynecol Obstet Invest. 1989;27(3):118-21. doi: 10.1159/000293635.

DOI:10.1159/000293635

PMID:2737543

Abstract

Ring preparations of human stem villous arteries were prepared by microtechnique from placentae born after normal-term vaginal delivery. Isometric tension was recorded in organ baths. Indomethacin 10(-8)-10(-5) M left responses to K+ depolarization and contractions produced by prostaglandin F2 alpha (PGF2 alpha) unaffected, while indomethacin 10(-4) M slightly decreased K+-induced contractions and inhibited responses to PGF2 alpha. In contrast, nitrendipine 10(-9)-10(-6) M markedly decreased responses to K+ while nitrendipine 10(-8)-10(-6) M inhibited PGF2 alpha-induced responses. Relaxant responses to vasointestinal polypeptide 10(-8)-10(-7)M were left unaffected by pretreatment with indomethacin 5 X 10(-5) M. Indomethacin affects contractile activation in human stem villous arteries only in high concentrations, and the effects differ in profile from those of calcium entry blockers like nitrendipine. The results do not leave evidence for increased placental arterial tone during indomethacin treatment in pregnancy.

摘要

采用显微技术从足月阴道分娩后的胎盘制备人绒毛干动脉的环状标本。在器官浴槽中记录等长张力。10⁻⁸ - 10⁻⁵ M的吲哚美辛对K⁺去极化反应以及前列腺素F2α（PGF2α）引起的收缩反应无影响，而10⁻⁴ M的吲哚美辛使K⁺诱导的收缩稍有减弱，并抑制对PGF2α的反应。相反，10⁻⁹ - 10⁻⁶ M的尼群地平显著减弱对K⁺的反应，而10⁻⁸ - 10⁻⁶ M的尼群地平抑制PGF2α诱导的反应。10⁻⁸ - 10⁻⁷ M的血管活性肠肽引起的舒张反应不受5×10⁻⁵ M吲哚美辛预处理的影响。吲哚美辛仅在高浓度时影响人绒毛干动脉的收缩激活，其作用模式与尼群地平这类钙通道阻滞剂不同。结果未发现孕期使用吲哚美辛治疗期间胎盘动脉张力增加的证据。