血管活性肠肽和P物质对足月妊娠人子宫肌层内动脉和绒毛干动脉的影响。

Effects of vasoactive intestinal polypeptide and substance P on human intramyometrial arteries and stem villous arteries in term pregnancy.

作者信息

Hansen V, Maigaard S, Allen J, Forman A

机构信息

Department of Obstetrics and Gynaecology, University of Aarhus, Denmark.

出版信息

Placenta. 1988 Sep-Oct;9(5):501-6. doi: 10.1016/0143-4004(88)90022-7.

DOI:10.1016/0143-4004(88)90022-7

PMID:2464822

Abstract

The effects of vasoactive intestinal polypeptide (VIP) and substance P on isolated human intramyometrial arteries and fetal stem villous arteries obtained from term pregnant women were compared. Ring preparations of small intramyometrial arteries and fetal stem villous arteries obtained at caesarean section were mounted in organ baths, and isometric tension was recorded. None of the peptides affected resting tension. In intramyometrial arteries precontracted by vasopressin (2.8 x 10(-9) M) both substance P (10(-12) to 10(-8) M) and VIP (10(-8) to 10(-6) M) caused relaxation. In fetal stem villous arteries precontracted by prostaglandin F2 alpha (10(-5) M) cumulative addition of substance P (10(-11) to 10(-6) M) did not produce significant changes in tension as compared with controls, while addition of single doses produced moderate relaxation. VIP (10(-8) to 10(-6) M) induced relaxation with similar effects for the addition of cumulative and single doses. The responses to VIP and substance P remained unaffected after pretreatment by atropine (10(-6) M), propranolol (10(-6) M), and indomethacin (10(-6) M). The results support a role for VIP and substance P in the regulation of uteroplacental blood flow in term pregnancy.

摘要

比较了血管活性肠肽（VIP）和P物质对从足月孕妇获取的人子宫肌层内动脉和胎儿干绒毛动脉的作用。将剖宫产时获取的子宫肌层内小动脉和胎儿干绒毛动脉的环行标本安装在器官浴槽中，并记录等长张力。这些肽均不影响静息张力。在由血管加压素（2.8×10⁻⁹ M）预收缩的子宫肌层内动脉中，P物质（10⁻¹²至10⁻⁸ M）和VIP（10⁻⁸至10⁻⁶ M）均引起舒张。在由前列腺素F2α（10⁻⁵ M）预收缩的胎儿干绒毛动脉中，与对照组相比，P物质（10⁻¹¹至10⁻⁶ M）的累积添加未引起张力的显著变化，而单剂量添加则产生适度舒张。VIP（10⁻⁸至10⁻⁶ M）对累积添加和单剂量添加诱导的舒张具有相似作用。用阿托品（10⁻⁶ M）、普萘洛尔（10⁻⁶ M）和吲哚美辛（10⁻⁶ M）预处理后，对VIP和P物质的反应未受影响。结果支持VIP和P物质在足月妊娠子宫胎盘血流调节中起作用。