Zhang Lei, Chan Paul, Liu Zhong-Min, Hwang Ling-Ling, Lin Kuo-Chi, Chan Wing P, Leung Ting-Kai, Choy Cheuk Sing
Department of Radiology, Shanghai East Hospital, Tongji University, 1800 Yantai Road, Shanghai 200123, China.
Division of Cardiology, Department of Internal Medicine, Wan Fang Hospital, Taipei Medical University, No. 111, Sec. 3, Singlong Road, Taipei 116, Taiwan.
Evid Based Complement Alternat Med. 2016;2016:7230962. doi: 10.1155/2016/7230962. Epub 2016 Jun 7.
The purpose of this study is to determine the possible effect of photoluminescence of bioceramic (PLB) on ischemic cerebral infarction (stroke), by using an animal model of transient middle cerebral artery occlusion (MCAO). Sprague-Dawley rats were used to induce MCAO to block the origin of the left MCAO; three months later, the positive chronic stroke rats were selected by running tunnel maze; the MCAO rats with significant chronic stroke and neurological defects were used for treadmill experiments with varying speed settings to test their capability for restoration after muscular fatigue under conditions of with and without PLB irradiation. As a result, PLB irradiation could improve exercise completion rate and average running speed during slow and fast treadmill settings. After PLB irradiation, the selected MCAO rats successfully completed all the second-round treadmill exercises at the maximum speed setting, and they had better restoration from muscular fatigue. An in vitro cell study on astrocytes of rats by bioceramic irradiation further demonstrated increased intracellular nitric oxide. To explain these results, we suggest that cortical brain stimulation of microcirculation and enhancement of peripheral muscular activity are the main causes of the improved exercise performance in MCAO rats by PLB.
本研究的目的是通过使用短暂性大脑中动脉闭塞（MCAO）动物模型，确定生物陶瓷光致发光（PLB）对缺血性脑梗死（中风）的可能影响。使用Sprague-Dawley大鼠诱导MCAO以阻断左大脑中动脉的起源；三个月后，通过运行迷宫对慢性中风阳性大鼠进行筛选；将具有明显慢性中风和神经功能缺损的MCAO大鼠用于不同速度设置的跑步机实验，以测试它们在有和没有PLB照射的情况下肌肉疲劳后恢复的能力。结果，PLB照射可提高慢速和快速跑步机设置期间的运动完成率和平均跑步速度。PLB照射后，所选的MCAO大鼠成功以最大速度设置完成了第二轮所有跑步机运动，并且它们从肌肉疲劳中的恢复更好。通过生物陶瓷照射对大鼠星形胶质细胞进行的体外细胞研究进一步表明细胞内一氧化氮增加。为了解释这些结果，我们认为皮层脑微循环刺激和外周肌肉活动增强是PLB改善MCAO大鼠运动表现的主要原因。
