Center for Neuroscience Research, SMART Institute of Advanced Biomedical Science, Konkuk University School of Medicine, Seoul, Republic of Korea.
Neuroimmunomodulation. 2012;19(5):267-76. doi: 10.1159/000335547. Epub 2012 Mar 30.
The phototherapeutic effects of low level infrared laser irradiation (808 nm) on brain neuronal cell protection after stroke have been presented recently. We previously reported that 710-nm wavelength visible light (VIS) increases total lymphocyte counts in vivo, especially CD4(+) T lymphocytes. In this study, we investigated the effects of 710-nm VIS irradiation on neuronal protection and recovery correlating with cellular immunity in stroke rats.
Rats were subjected to 90-min middle cerebral artery occlusion (MCAO) followed by reperfusion and were divided into two groups: irradiation and no irradiation. The irradiation group had been exposed to 710-nm VIS for 3 weeks after MCAO establishment or sham operation. The helper T cell (CD4(+)) count in the whole blood and infarct volume were measured. Messenger RNA expression levels of IL-4 and IL-10 in peripheral blood mononuclear cells were measured, a histologic study including microglia activation and regulatory T (Treg) cell markers, neurological severity scoring and a parallel bar walking test were all performed.
CD4(+) cell count was reduced after MCAO but was significantly increased by 710-nm VIS irradiation. The infarct sizes were decreased in the MCAO + irradiation group compared with the MCAO control group. IL-10 mRNA expression and the immunoreactivity of Treg cells were increased in the MCAO + irradiation group compared with the MCAO control group. Increased microglia activation after MCAO was reduced by 710-nm VIS irradiation. The irradiation group also showed improved neurological severity score levels and step fault scores after MCAO.
Our data suggest that 710-nm VIS irradiation may activate cellular immunity, reduce brain infarction and ultimately induce functional recovery in a stroke animal model.
最近有研究报道，低强度红外激光辐照（808nm）对脑卒中后脑神经元细胞具有光疗保护作用。我们之前曾报道过，710nm 波长可见光（VIS）可增加体内总淋巴细胞计数，尤其是 CD4+T 淋巴细胞。在这项研究中，我们研究了 710nm VIS 辐照对脑卒中大鼠神经元保护和恢复的影响，以及与细胞免疫的相关性。
大鼠接受 90 分钟大脑中动脉闭塞（MCAO），随后再灌注，并分为两组：辐照组和非辐照组。辐照组在 MCAO 建立或假手术后接受 710nm VIS 照射 3 周。测量全血中辅助性 T 细胞（CD4+）计数和梗死体积。测量外周血单个核细胞中白细胞介素 4（IL-4）和白细胞介素 10（IL-10）的信使 RNA 表达水平，进行组织学研究，包括小胶质细胞激活和调节性 T（Treg）细胞标志物，进行神经功能严重程度评分和平行棒行走试验。
MCAO 后 CD4+细胞计数减少，但 710nm VIS 照射可显著增加。MCAO+辐照组的梗死体积较 MCAO 对照组减小。MCAO+辐照组的 IL-10 mRNA 表达和 Treg 细胞免疫反应性较 MCAO 对照组增加。MCAO 后增加的小胶质细胞激活被 710nm VIS 照射减少。照射组在 MCAO 后神经功能严重程度评分和步差错评分也有所改善。
我们的数据表明，710nm VIS 照射可能激活细胞免疫，减少脑梗死，最终在脑卒中动物模型中诱导功能恢复。
