Ogura Yusuke, Onishi Atsushi, Nishimura Takahiro, Tanida Jun
Graduate School of Information Science and Technology, Osaka University, Suita, Osaka, 5650871, Japan.
Biomed Opt Express. 2016 May 9;7(6):2142-53. doi: 10.1364/BOE.7.002142. eCollection 2016 Jun 1.
Optically controlled release of a DNA strand based on a nonradiative relaxation process of black hole quenchers (BHQs), which are a sort of dark quenchers, is presented. BHQs act as efficient energy sources because they relax completely via a nonradiative process, i.e., without fluorescent emission-based energy losses. A DNA strand is modified with BHQs and the release of its complementary strand is controlled by excitation of the BHQs. Experimental results showed that up to 50% of the target strands were released, and these strands were capable of inducing subsequent reactions. The controlled release was localized on a substrate within an area of no more than 5 micrometers in diameter.
本文介绍了基于黑洞猝灭剂（BHQs，一种暗猝灭剂）的非辐射弛豫过程实现DNA链的光控释放。BHQs作为高效的能量源，因为它们通过非辐射过程完全弛豫，即没有基于荧光发射的能量损失。用BHQs修饰DNA链，其互补链的释放通过BHQs的激发来控制。实验结果表明，高达50%的目标链被释放，并且这些链能够引发后续反应。可控释放在直径不超过5微米的基底区域内实现。
