Frontiers Science Center for Flexible Electronics, Xi'an Institute of Flexible Electronics (IFE) and Xi'an Institute of Biomedical Materials & Engineering (IBME), Northwestern Polytechnical University, Xi'an, 710072, China.
State Key Laboratory of Solidification Processing, School of Materials Science and Engineering, Northwestern Polytechnical University, 127 West Youyi Road, Xi'an, 710072, China.
Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202207188. doi: 10.1002/anie.202207188. Epub 2022 Sep 8.
For fluorogenic probes, Förster resonance energy transfer (FRET) is one of the most widely used mechanisms to realize the detection of biochemical activities. Dark quenchers relax from the excited state to the ground state non-radiatively, and are promising alternatives to fluorescent FRET acceptors. Small-molecule (dark) quenchers have been widely used as acceptors in FRET-based probes to monitor various physiological processes with minimum background signal. Herein, we summarize the relevant advances of small-molecule quenchers that are used in FRET-based probes. This Review is intended to provide suggestions regarding the rationale design and selection of appropriate fluorophore-quencher FRET pairs, which are fully compatible with challenging analytical applications in various biological systems. Finally, an outlook of the future biomedical applications and developments of this field is presented.
对于荧光探针来说，Förster 共振能量转移（FRET）是实现生化活性检测的最广泛应用的机制之一。暗猝灭剂从激发态非辐射地弛豫到基态，是荧光 FRET 受体的有前途的替代品。小分子（暗）猝灭剂已广泛用作基于 FRET 的探针中的受体，以最小的背景信号监测各种生理过程。在此，我们总结了用于 FRET 探针的小分子猝灭剂的相关进展。本综述旨在提供有关合理设计和选择合适的荧光团-猝灭剂 FRET 对的建议，这些建议与各种生物系统中具有挑战性的分析应用完全兼容。最后，提出了该领域未来生物医学应用和发展的展望。
