Hashieh I A, Rémy L, Mathieu S, Gérolami A
INSERM U260, Faculté de Médecine, Marseille, France.
Hepatology. 1989 Jul;10(1):61-5. doi: 10.1002/hep.1840100113.
A kinetic study of horseradish peroxidase transport was carried out on short-term cultured adult rat hepatocytes. After 4 hr of plating, cells were preincubated with monensin (1 microM) for 1 hr before their incubation with horseradish peroxidase for different times. Monensin treatment resulted in the dilatation of the Golgi apparatus and caused the appearance of numerous intracellular lumina lined with microvilli in associated as well as isolated hepatocytes but did not modify newly formed bile canaliculi. The frequency of their appearance increased to 28% in cells pretreated with monensin compared to 2% in controls. Intracellular lumina membranes had the morphological features of apical membranes and were stained with horseradish peroxidase more frequently than those of newly formed bile canaliculi. This work therefore provides a model for studying bile secretion in cultured hepatocytes. Our results also suggest that the biliary transport of horseradish peroxidase does not involve the Golgi apparatus, since horseradish peroxidase was never observed in the Golgi stacks even after monensin treatment.
对短期培养的成年大鼠肝细胞进行了辣根过氧化物酶转运的动力学研究。接种4小时后，细胞在与辣根过氧化物酶孵育不同时间之前，先用莫能菌素（1微摩尔）预孵育1小时。莫能菌素处理导致高尔基体扩张，并在相关联的和孤立的肝细胞中引起许多内衬微绒毛的细胞内腔的出现，但未改变新形成的胆小管。与对照组的2%相比，在用莫能菌素预处理的细胞中，其出现频率增加到28%。细胞内腔膜具有顶端膜的形态特征，并且比新形成的胆小管的膜更频繁地被辣根过氧化物酶染色。因此，这项工作为研究培养肝细胞中的胆汁分泌提供了一个模型。我们的结果还表明，辣根过氧化物酶的胆汁转运不涉及高尔基体，因为即使在莫能菌素处理后，在高尔基体堆栈中也从未观察到辣根过氧化物酶。
