Ribeiro Alves Márcia Regina, Zuñiga Abraham Damian Giraldo, Sousa Rita de Cássia Superbi, Zacchi Scolforo Carmelita
Biodiversity Network and Biotechnology Legal-Bionorte Amazon, Federal University of Tocantins, 109 Norte Avenida NS-15, ALCNO-14, Plan Director North, 77001-090 Palmas, TO, Brazil.
Department of Chemistry, Federal University of Viçosa, Avenida PH Holfs, 36570-000 Viçosa, MG, Brazil.
ScientificWorldJournal. 2016;2016:2808241. doi: 10.1155/2016/2808241. Epub 2016 Jun 7.
Bovine serum albumin is one of the major serum proteins; it plays an important role as a result of its functional and nutritional properties which have bioactive peptides. Adsorption method was used to separate protein, which involves hydroxyapatite, synthetic hydroxyapatite, and active babassu coal. Initially, characterization was carried out using the zeta potential of the adsorbents. Kinetic pseudo-first- and pseudo-second-order models were applied. For isotherms, equilibrium data studies were carried out using the Langmuir and Freundlich models, in addition to determining the efficiency of adsorptive process. The results of the zeta potential showed loads ranging from +6.9 to -42.8 mV. The kinetic data were better represented in the pseudo-second-order model with chemisorption characteristics. The adsorption capacity of the adsorbents decreased as pH increased, indicating that the electrostatic bonds and some functional groups of active babassu coal contributed to the reduction of adsorption, especially oxygen linked to carbon atoms. The value of pH 4.0 showed the best results of adsorption, being obtained as the maximum adsorption capacity (q m ) and yield (%) (where q m = 87.95 mg g(-1) and 74.2%; 68.26 mg g(-1) and 68.6%; and 36.18 mg g(-1), 37.4%) of hydroxyapatite, synthetic hydroxyapatite, and active babassu coal, respectively.
牛血清白蛋白是主要的血清蛋白之一；由于其具有生物活性肽的功能和营养特性，它发挥着重要作用。采用吸附法分离蛋白质，该方法涉及羟基磷灰石、合成羟基磷灰石和活性巴巴苏煤。最初，使用吸附剂的zeta电位进行表征。应用了动力学伪一级和伪二级模型。对于等温线，除了确定吸附过程的效率外，还使用朗缪尔模型和弗伦德里希模型进行平衡数据研究。zeta电位的结果显示负载范围为+6.9至-42.8 mV。动力学数据在具有化学吸附特征的伪二级模型中表现得更好。吸附剂的吸附容量随着pH值的升高而降低，这表明活性巴巴苏煤的静电键和一些官能团有助于吸附的降低，尤其是与碳原子相连的氧。pH值为4.0时显示出最佳的吸附结果，分别获得了羟基磷灰石、合成羟基磷灰石和活性巴巴苏煤的最大吸附容量（qm）和产率（%）（其中qm = 87.95 mg g(-1)和74.2%；68.26 mg g(-1)和68.6%；以及36.18 mg g(-1)，37.4%）。
