Liu Qianchun, Lan Yanping, Wen Changlong, Zhao Hong, Wang Jian, Wang Yongqin
Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing 100097, China.
Institute of Agricultural Integrated Development, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Int J Mol Sci. 2016 Jul 1;17(7):1058. doi: 10.3390/ijms17071058.
Cytoplasmic male sterility (CMS) is important for exploiting heterosis in crop plants and also serves as a model for investigating nuclear-cytoplasmic interaction. The molecular mechanism of cytoplasmic male sterility and fertility restoration was investigated in several important economic crops but remains poorly understood in the Welsh onion. Therefore, we compared the differences between the CMS line 64-2 and its maintainer line 64-1 using transcriptome sequencing with the aim of determining critical genes and pathways associated with male sterility. This study combined two years of RNA-seq data; there were 1504 unigenes (in May 2013) and 2928 unigenes (in May 2014) that were differentially expressed between the CMS and cytoplasmic male maintainer Welsh onion varieties. Known CMS-related genes were found in the set of differentially expressed genes and checked by qPCR. These genes included F-type ATPase, NADH dehydrogenase, cytochrome c oxidase, etc. Overall, this study demonstrated that the CMS regulatory genes and pathways may be associated with the mitochondria and nucleus in the Welsh onion. We believe that this transcriptome dataset will accelerate the research on CMS gene clones and other functional genomics research on A. fistulosum L.
细胞质雄性不育（CMS）对于作物杂种优势的利用非常重要，同时也作为研究核质相互作用的模型。在几种重要的经济作物中对细胞质雄性不育和育性恢复的分子机制进行了研究，但在大葱中仍了解甚少。因此，我们利用转录组测序比较了CMS系64-2与其保持系64-1之间的差异，目的是确定与雄性不育相关的关键基因和途径。本研究结合了两年的RNA测序数据；在CMS和细胞质雄性保持大葱品种之间有1504个单基因（2013年5月）和2928个单基因（2014年5月）差异表达。在差异表达基因集中发现了已知的与CMS相关的基因，并通过qPCR进行了验证。这些基因包括F型ATP酶、NADH脱氢酶、细胞色素c氧化酶等。总体而言，本研究表明CMS调控基因和途径可能与大葱的线粒体和细胞核有关。我们相信这个转录组数据集将加速大葱CMS基因克隆及其他功能基因组学研究。
