Mahmoodzadeh Hosseini Hamideh, Kiyani Nasibeh, Amin Mohsen, Hedayati Ch Mojtaba, Imani Fooladi Abbas Ali
a Applied Microbiology Research Center , Baqiyatallah University of Medical Sciences , Tehran , Iran.
b Faculty of Biotechnology, Branch Tehran-Shargh , Payame Noor University , Tehran , Iran.
J Chemother. 2017 Aug;29(4):215-219. doi: 10.1080/1120009X.2016.1201257. Epub 2016 Jul 4.
Mupirocin is a topical antibiotic for methicillin-resistant Staphylococcus aureus (MRSA) decolonization in hospital settings and nursing homes and is used as a highly effective antibiotic against MRSA. In this study, we aimed to evaluate the frequency of high-level mupirocin-resistant (HLMR) strains among the MRSA subtypes. A total of 188 clinical MRSA isolates were collected from 2011 to 2014, and their susceptibility to antimicrobial agents and vancomycin resistance was evaluated using disc diffusion method and micro-dilution method, respectively. Furthermore, the presence of mecA, SSCmec, mupA and mupB was assessed by PCR. All isolates were multi-drug resistant (MDR) but 2 strains (1.06%) were resistant to mupirocin. Minimum inhibitory concentration (MIC) of vancomycin for 8 strains (4.7%) was higher than 2 μg/ml. Of 188 isolates, 188 (100%), 64 (34.04%), 8 (4.3%), 150 (79.8%), 26 (13.8%), 2 (1.06) and 2 (1.06%) isolates possessed mecA, SCCmec types I, II, III, IV, mupA and mupB genes, respectively. Our data showed that despite infection control policy enforced by health care committee, the rate of mupirocin resistance among MRSA strains is continuously rising.
莫匹罗星是一种用于医院环境和疗养院中耐甲氧西林金黄色葡萄球菌（MRSA）去定植的外用抗生素，并且用作对抗MRSA的高效抗生素。在本研究中，我们旨在评估MRSA亚型中高水平莫匹罗星耐药（HLMR）菌株的频率。2011年至2014年共收集了188株临床MRSA分离株，分别使用纸片扩散法和微量稀释法评估它们对抗菌药物的敏感性和对万古霉素的耐药性。此外，通过PCR评估mecA、SSCmec、mupA和mupB的存在情况。所有分离株均为多重耐药（MDR），但有2株（1.06%）对莫匹罗星耐药。8株（4.7%）分离株的万古霉素最低抑菌浓度（MIC）高于2μg/ml。在188株分离株中，分别有188株（100%）、64株（34.04%）、8株（4.3%）、150株（79.8%）、26株（13.8%）、2株（1.06%）和2株（1.06%）分离株分别携带mecA、I型、II型、III型、IV型SSCmec、mupA和mupB基因。我们的数据显示，尽管医疗保健委员会实施了感染控制政策，但MRSA菌株中莫匹罗星耐药率仍在持续上升。
