Awaad Aziz, Moustafa Alaa Y
Department of Zoology, Faculty of Science, Sohag University, Sohag 82524, Egypt.
Department of Zoology, Faculty of Science, Sohag University, Sohag 82524, Egypt.
Acta Histochem. 2016 Jul;118(6):596-605. doi: 10.1016/j.acthis.2016.06.005. Epub 2016 Jul 1.
Acid secretion containing sulfuric and hydrochloric acids is a fascinating defensive phenomenon within many groups of marine organisms. This study aimed to investigate the mice spleen histology and immunotoxicity using skin acid secretion (SAS) of the sea slug Berthellina citrina after oral administration. The spleen showed atrophy in the white pulp, decrease in the splenocytes density, megakaryocytes cytoplasmic degeneration as well as inflammatory cells infiltrations. The white and red pulp splenocytes number decreased time-dependently in the treated spleens. Additionally, the size of the megakaryocytes increased as compared with the control. The administration with SAS increased the number of the IgA(+) cells aggregation in the splenic red pulp. Furthermore, after 7days of the administration, large number of dispersed IgA(+) cells were distributed in splenic parenchyma. The IgA(+) cells numbers increased time-dependently as compared with those in the control. The aggregation sizes and number of the F4/80(+) cell in the splenic red pulp were increased. Furthermore the F4/80(+) cells numbers increased time-dependently as compared with those in the control. The UEAI(+) cells were found as free cells but not in aggregations in the control splenic red pulp. Contradictory to the number of IgA(+) cells and F4/80(+) cells the number of the UEAI(+) cells decreased time-dependently after administration with SAS. Hematologically, abnormal numbers of WBCs different cells were observed after administration with SAS. This study provides new insight about the toxicity of a marine extract may be used in natural products industry or medical applications.
含有硫酸和盐酸的酸分泌是许多海洋生物群体中一种引人入胜的防御现象。本研究旨在通过口服海蛞蝓柠檬背海兔的皮肤酸分泌（SAS）来研究小鼠脾脏组织学和免疫毒性。脾脏显示白髓萎缩、脾细胞密度降低、巨核细胞胞质变性以及炎症细胞浸润。在处理过的脾脏中，白髓和红髓脾细胞数量随时间呈依赖性减少。此外，与对照组相比，巨核细胞的大小增加。给予SAS后，脾脏红髓中IgA（+）细胞聚集数量增加。此外，给药7天后，大量分散的IgA（+）细胞分布在脾实质中。与对照组相比，IgA（+）细胞数量随时间呈依赖性增加。脾脏红髓中F4/80（+）细胞的聚集大小和数量增加。此外，与对照组相比，F4/80（+）细胞数量随时间呈依赖性增加。在对照脾脏红髓中，UEAI（+）细胞以游离细胞形式存在，而非聚集形式。与IgA（+）细胞和F4/80（+）细胞数量相反，给予SAS后，UEAI（+）细胞数量随时间呈依赖性减少。血液学方面，给予SAS后观察到不同类型白细胞数量异常。本研究为一种海洋提取物的毒性提供了新的见解，该提取物可能用于天然产品行业或医学应用。
