Strand Nicholas S, Hoi Kimberly K, Phan Tien M T, Ray Catherine A, Berndt Jason D, Moon Randall T
Department of Pharmacology, Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, WA, 98195, USA; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA, 98195, USA.
Department of Pharmacology, Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, WA, 98195, USA; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA, 98195, USA.
Biochem Biophys Res Commun. 2016 Sep 2;477(4):952-956. doi: 10.1016/j.bbrc.2016.07.006. Epub 2016 Jul 4.
Unlike mammals, zebrafish can regenerate their injured spinal cord and regain control of caudal tissues. It was recently shown that Wnt/β-catenin signaling is necessary for spinal cord regeneration in the larval zebrafish. However, the molecular mechanisms of regeneration may or may not be conserved between larval and adult zebrafish. To test this, we assessed the role of Wnt/β-catenin signaling after spinal cord injury in the adult zebrafish. We show that Wnt/β-catenin signaling is increased after spinal cord injury in the adult zebrafish. Moreover, overexpression of Dkk1b inhibited Wnt/β-catenin signaling in the regenerating spinal cord of adult zebrafish. Dkk1b overexpression also inhibited locomotor recovery, axon regeneration, and glial bridge formation in the injured spinal cord. Thus, our data illustrate a conserved role for Wnt/β-catenin signaling in adult and larval zebrafish spinal cord regeneration.
与哺乳动物不同，斑马鱼能够再生其受损的脊髓并重新恢复对尾部组织的控制。最近的研究表明，Wnt/β-连环蛋白信号通路对幼体斑马鱼的脊髓再生是必需的。然而，幼体和成年斑马鱼之间再生的分子机制可能保守，也可能不保守。为了验证这一点，我们评估了成年斑马鱼脊髓损伤后Wnt/β-连环蛋白信号通路的作用。我们发现成年斑马鱼脊髓损伤后Wnt/β-连环蛋白信号通路增强。此外，Dkk1b的过表达抑制了成年斑马鱼再生脊髓中的Wnt/β-连环蛋白信号通路。Dkk1b的过表达还抑制了受损脊髓中的运动恢复、轴突再生和胶质桥形成。因此，我们的数据说明了Wnt/β-连环蛋白信号通路在成年和幼体斑马鱼脊髓再生中具有保守作用。
